CAJ | 학술논문

目的分析不同核苷(酸)类似物治疗的乙型肝炎病毒感染者乙型肝炎病毒P基因逆转录酶区变异特点.方法采用直接测序法对471例慢性乙型肝炎病毒感染者乙型肝炎病毒P基因逆转录酶区进行测序,比较未曾应用核苷(酸)类似物(第1组)、曾经应用核苷(酸)类似物停药(第2组)和正在应用核苷(酸)类似物(第3组)治疗患者基因突变的特点.结果在第1组、第2组和第3组中分别检出耐药相关错义变异6例(2.0%)、4例(8.2%)和50例(38.5%),同义变异分别为25例(8.5%)、5例(10.2%)和20例(15.4%);在第3组患者中,错义变异组、同义变异组和未变异组血清HBV DNA分别为5.58±1.36lgcopies/ml、5.27±1.05lgcopies/ml和4.98±1.50 lgcopies/ml,差异无统计学意义(P>0.05).结论不同核苷(酸)类似物治疗的乙型肝炎病毒感染者体内病毒耐药变异株存在的意义还需要进一步研究.
목적분석불동핵감(산)유사물치료적을형간염병독감염자을형간염병독P기인역전록매구변이특점.방법채용직접측서법대471례만성을형간염병독감염자을형간염병독P기인역전록매구진행측서,비교미증응용핵감(산)유사물(제1조)、증경응용핵감(산)유사물정약(제2조)화정재응용핵감(산)유사물(제3조)치료환자기인돌변적특점.결과재제1조、제2조화제3조중분별검출내약상관착의변이6례(2.0%)、4례(8.2%)화50례(38.5%),동의변이분별위25례(8.5%)、5례(10.2%)화20례(15.4%);재제3조환자중,착의변이조、동의변이조화미변이조혈청HBV DNA분별위5.58±1.36lgcopies/ml、5.27±1.05lgcopies/ml화4.98±1.50 lgcopies/ml,차이무통계학의의(P>0.05).결론불동핵감(산)유사물치료적을형간염병독감염자체내병독내약변이주존재적의의환수요진일보연구.
Objective To investigate the mutation of hepatitis B viral polymerase motif in patients with chronic hepatitis B receiving nucleos (t)ides treatment. Method The HBV polymerase reverse transcriptase genes in 481 patients with chronic hepatitis B infection was analysed by direct sequencing. The patients were divided into 3 groups:never applying to nucleoside analogue(NA,group 1),applying to NA before (group 2) and applying to NA at the moment of study (group 3). Results The missense variations in group 1,2 and 3 were found in 6 (2.0%),4(8.2%) and 50(38.5%),while the homonymy variations in group 1,2 and 3 were found in 25(8.5%),5 (10.2%)and 20 (15.4%),respectively;Out of group 3, the serum HBV DNA levels were 5.58±1.36lgcopies/ml in patients with missense variation,5.27 ±1.05lgcopies/ml in with homonymy variation and 4.98 ±1.50lgcopies/ml in without variation,respectively (P>0.05). Conclusion Further investigation of HBV variation may help solve the problem of drug resistance in patients with hepatitis B.