CAJ | 학술논문

GPD1是3–磷酸甘油脱氢酶的编码基因,在酵母的耐盐通路中发挥着重要的作用.为了探讨耐盐酵母家族成员鲁氏酵母的 GPD1对酿酒酵母的影响,本文利用分子生物学方法构建了质粒 YEplac195-GPD1,并将其转入到酿酒酵母菌株W303中,得到工程菌株WYS.研究发现在菌株WYS中过量表达鲁氏酵母的GPD1,其耐盐性、抗性及甘油产量均明显高于对照菌株WY,特别是在含盐量为18%的情况下菌株WYS甘油产量提高了14.35%.说明GPD1的过量表达是菌株耐盐性提高的重要原因.
GPD1시3–린산감유탈경매적편마기인,재효모적내염통로중발휘착중요적작용.위료탐토내염효모가족성원로씨효모적 GPD1대양주효모적영향,본문이용분자생물학방법구건료질립 YEplac195-GPD1,병장기전입도양주효모균주W303중,득도공정균주WYS.연구발현재균주WYS중과량표체로씨효모적GPD1,기내염성、항성급감유산량균명현고우대조균주WY,특별시재함염량위18%적정황하균주WYS감유산량제고료14.35%.설명GPD1적과량표체시균주내염성제고적중요원인.
GPD1 encoded glycerol-3-phosphate dehydrogenase plays an important role in the salt-tolerant pathway of yeast. Zygosaccharomyces rouxi belongs to the salt-tolerant family. In order to analyze the effect of ZrGPD1(GPD1 gene of Zygo-saccharomyces rouxi)on the salt-tolerance of Saccharomyces cerevisiae,plasmid YEplac195-GPD1 was constructed and then transformed into Saccharomyces cerevisiae W303,forming the engineered strain WYS. The results showed the the salt tolerance,resistance and the production of glycerol in the strain WYS were improved by overexpression of GPD1 compared with that of the control strain WY. Noticeably,the production of glycerol in the strain WYS was raised by 14.35%under the condition of 18%salt. The data suggested that overexpression of GPD1 was the important reason for the improvement of salt tolerance.