生态环境学报
生態環境學報
생태배경학보
ECOLOGY AND ENVIRONMENT
2012年
11期
1830-1835
,共6页
阮新民%施伏芝%罗志祥*
阮新民%施伏芝%囉誌祥*
원신민%시복지%라지상*
水稻%开花期%生理生化性状%氮肥利用率
水稻%開花期%生理生化性狀%氮肥利用率
수도%개화기%생리생화성상%담비이용솔
rice%flowering stage%physiological and biochemical characteristics%NUE
选用氮高效水稻 Dasanbyeo 与氮低效水稻 Lemont,采用土培方法,在4个施氮量(0、1.04、1.55、2.07 g?盆-1)处理下,主要研究了开花期不同氮素利用率水稻品种的碳氮代谢生化特性及与氮肥利用率的关系.结果表明,在开花期的氮高效品种 Dasanbyeo 磷酸烯醇式丙酮酸羧化酶(PEPCase)、二磷酸核酮糖羧化酶(RuBPCase)、蔗糖磷酸合成酶(SPS)、硝酸还原酶(NR)及蛋白质(Pro)含量均明显高于氮低效品种 Lemont;且在中、低氮(1.03~2.07 g?盆-1)水平时品种间差异显著.2种不同氮效率水稻碳氮代谢调节酶 RuBPCase、SPS、NR 活性均随施氮量的增加而呈抛物线增加.氮高效品种 PEPCase酶活性与 Pro 含量随施氮量的增加而呈线性增加;品种间净光合速率(Pn)在不同氮素水平差异不显著.回归分析表明, NR、PEPCase 活性与 Pro 含量是导致氮肥利用率(NUE)品种间差异的主要生理表现.
選用氮高效水稻 Dasanbyeo 與氮低效水稻 Lemont,採用土培方法,在4箇施氮量(0、1.04、1.55、2.07 g?盆-1)處理下,主要研究瞭開花期不同氮素利用率水稻品種的碳氮代謝生化特性及與氮肥利用率的關繫.結果錶明,在開花期的氮高效品種 Dasanbyeo 燐痠烯醇式丙酮痠羧化酶(PEPCase)、二燐痠覈酮糖羧化酶(RuBPCase)、蔗糖燐痠閤成酶(SPS)、硝痠還原酶(NR)及蛋白質(Pro)含量均明顯高于氮低效品種 Lemont;且在中、低氮(1.03~2.07 g?盆-1)水平時品種間差異顯著.2種不同氮效率水稻碳氮代謝調節酶 RuBPCase、SPS、NR 活性均隨施氮量的增加而呈拋物線增加.氮高效品種 PEPCase酶活性與 Pro 含量隨施氮量的增加而呈線性增加;品種間淨光閤速率(Pn)在不同氮素水平差異不顯著.迴歸分析錶明, NR、PEPCase 活性與 Pro 含量是導緻氮肥利用率(NUE)品種間差異的主要生理錶現.
선용담고효수도 Dasanbyeo 여담저효수도 Lemont,채용토배방법,재4개시담량(0、1.04、1.55、2.07 g?분-1)처리하,주요연구료개화기불동담소이용솔수도품충적탄담대사생화특성급여담비이용솔적관계.결과표명,재개화기적담고효품충 Dasanbyeo 린산희순식병동산최화매(PEPCase)、이린산핵동당최화매(RuBPCase)、자당린산합성매(SPS)、초산환원매(NR)급단백질(Pro)함량균명현고우담저효품충 Lemont;차재중、저담(1.03~2.07 g?분-1)수평시품충간차이현저.2충불동담효솔수도탄담대사조절매 RuBPCase、SPS、NR 활성균수시담량적증가이정포물선증가.담고효품충 PEPCase매활성여 Pro 함량수시담량적증가이정선성증가;품충간정광합속솔(Pn)재불동담소수평차이불현저.회귀분석표명, NR、PEPCase 활성여 Pro 함량시도치담비이용솔(NUE)품충간차이적주요생리표현.
The relationship between the biochemical characteristics and nitrogen use efficiency(NUE) in the Flowering Stage of the rice (Oryza Sativa L.) variety-Dasanbyeo with high NUE and Lemont with low NUE, which were treated with 4 nitrogen-applying rates(0, 1.04, 1.55 and 2.07 g?pot-1)under the condition of pot culturation, was studied. The result indicated that the activity of phosphoenolpyruvate carboxylase (PEPCase), ribulose bisphosphate carboxylase (RuBPCase) and sucrose phosphorylase synthase (SPS) and the content of protein in the flowering stage of Dasanbyeo were obviously higher than those in Lemont and the difference in those indexes between the two different varieties were significant at the treatment with meddle and low nitrogen-applying rate(1.03-2.07 g?pot-1). The activity of RuBPCase, SPS and Nitrate reductase (NR) of the two species of rice varieties was varied with the increment of nitrogen-applying rate, which relationship was in the parabola in shape. The activity of PEPCase and the content of protein in Dasanbyeo were raised with the increment of nitrogen-applying rate, which showed a linear relation. The difference of net photosynthesis rate between the two different varieties were not significant at the difference treatments of nitrogen level. The regression analysis showed that the difference in the activity of NR, PEPCase and the content of Pro resulted mainly in the various phenotypes of NUE of different varieties.