CAJ | 학술논문

自然界中很多种类的植物与固氮微生物共生,故其自身可进行氮同化,尤以豆科植物为甚.豆科植物由于与固氮微生物共生,所以豆科植物根际土壤的氮素较高于非固氮类植物种类.由于氮素是微生物代谢必需的营养元素之一,所以,豆科植物与非豆科植物的根际微环境存在一定的差异,自然而然两者根际土壤微生物的种群数量及其代谢活性也应存在一定的差异.基于此,本研究拟选取两种豆科植物(合欢Albizia julibrissin和豌豆Pisum sativum)及两种非豆科植物(樱花Prunus serrulata和艾蒿Artemisia argyi)的根际土壤微生物为研究对象,以平板梯度稀释法测定其种群数量,并通过测定纤维素酶、转化酶、脲酶、硝酸还原酶和酸性磷酸酶的活性为切入点分析其代谢活性,进而分析豆科植物对根际土壤微生物种群数量及其代谢的影响.本研究结果显示豆科植物的根际土壤细菌和真菌数量显著低于非豆科植物的.木本非豆植物和木本豆科植物根际土壤放线菌数量无显著差异,但是草本豆科植物根际土壤放线菌数量却显著高于草本非豆科植物的.另外,豆科植物根际土壤纤维素酶、转化酶、硝酸还原酶和酸性磷酸酶活性均显著大于非豆科植物的,而豆科植物根际土壤脲酶活性却显著低于非豆科植物的.另外,转化酶和脲酶活性与真菌数量呈显著相关.
자연계중흔다충류적식물여고담미생물공생,고기자신가진행담동화,우이두과식물위심.두과식물유우여고담미생물공생,소이두과식물근제토양적담소교고우비고담류식물충류.유우담소시미생물대사필수적영양원소지일,소이,두과식물여비두과식물적근제미배경존재일정적차이,자연이연량자근제토양미생물적충군수량급기대사활성야응존재일정적차이.기우차,본연구의선취량충두과식물(합환Albizia julibrissin화완두Pisum sativum)급량충비두과식물(앵화Prunus serrulata화애호Artemisia argyi)적근제토양미생물위연구대상,이평판제도희석법측정기충군수량,병통과측정섬유소매、전화매、뇨매、초산환원매화산성린산매적활성위절입점분석기대사활성,진이분석두과식물대근제토양미생물충군수량급기대사적영향.본연구결과현시두과식물적근제토양세균화진균수량현저저우비두과식물적.목본비두식물화목본두과식물근제토양방선균수량무현저차이,단시초본두과식물근제토양방선균수량각현저고우초본비두과식물적.령외,두과식물근제토양섬유소매、전화매、초산환원매화산성린산매활성균현저대우비두과식물적,이두과식물근제토양뇨매활성각현저저우비두과식물적.령외,전화매화뇨매활성여진균수량정현저상관.
Many plant species build the mutualism relationship with nitrogen-fixing microorganisms in nature. Thus, those plant species have the functioning of nitrogen fixation, especially leguminous plant species. According to logical thinking, the concentration of nitrogen nutrient in rhizospheral soils of leguminous plant species were higher than that of non-leguminous plant species. Thus, there may be different in the microenvironment of rhizospheral soils as well as the amount and metabolic activities of microorganisms in rhizospheral soils between leguminous plants and non-leguminous plant species inferentially due to the fact that nitrogen nutrient was the essential elements of microbial metabolism. Thus, this study was carried out with two leguminous plant species (woody:Albizia julibrissin and herbal:Pisum sativum) and two non-leguminous plant species (woody:Prunus serrulata and herbal: Artemisia argyi) seleseted as research target in order to assess (1) the effects of leguminous plant species on metabolic amount in rhizospheral soils revealed by dilution plate method with spread plates, and (2) the effects of leguminous plant species on the metabolic activities of microorganisms in rhizospheral soils through determine soil enzyme activities (i.e., cellulase, invertase, urease, nitrate reductase, and acid phosphatase) to insights into the effects of leguminous plant species on soil microorganism community. The results of this study suggested that the amount of bacterial and fungal in rhizospheral soils of the two leguminous plant species were significantly lower than those of non-leguminous plant species. The actinomycetic amount in rhizospheral soils of two woody species were equivalent basically, while the actinomycetic amount in rhizospheral soils of herbal leguminous plant species were significantly higher than those of herbal non-leguminous plant species. Meanwhile, the activities of cellulase, invertase, nitrate reductase, and acid phosphatase in rhizospheral soils of leguminous plant species were significantly higher than those of non-leguminous plant species. But, an interesting phenomenon was that urease activities in rhizospheral soils of non-leguminous plant species were higher than those of leguminous plant species significantly. The activities of invertase and urease were significantly correlated with fungal amount in rhizospheral soils of the four plant species.