CAJ | 학술논문

为研究运动激活的CaMK II对糖尿病大鼠骨骼肌MEF2和GLUT4结合活性及GLUT4基因表达量的调节作用,以了解运动提高糖尿病大鼠GLUT4基因表达的可能机制.将健康SD大鼠100只,40只作为正常对照,其余60只建立糖尿病大鼠模型,建模成功35只.正常对照和糖尿病大鼠再按体重各自随机分为5组:安静对照组、一次性运动组、一次性运动+KN93组、耐力训练组、耐力训练+KN93组,共10组.跑台速度20 m/min,运动时间1 h.一次性运动组大鼠1次运动后3 h取材.耐力训练组采用同样跑台速度和运动时间,训练1周,并于最后1次训练后12 h取材.结果得到,糖尿病大鼠耐力训练组,骨骼肌细胞核内MEF2和GLUT4的结合活性及骨骼肌 GLUT4基因表达量虽然均显著低于正常对照大鼠耐力训练组,但与糖尿病大鼠安静对照组相比均显著升高;耐力训练+KN93组,骨骼肌细胞核内MEF2和GLUT4的结合活性与安静对照组相比差异没有显著性,但骨骼肌 GLUT4基因表达量与安静对照组相比却显著升高.结果显示:虽然运动通过激活糖尿病大鼠骨骼肌CAMK II而提高其骨骼肌细胞核内MEF2和GLUT4的结合活性,虽然MEF2和GLUT4的结合活性参与调解了正常大鼠骨骼肌GLUT4基因表达,但并不是影响糖尿病大鼠骨骼肌GLUT4基因表达的唯一因素.
위연구운동격활적CaMK II대당뇨병대서골격기MEF2화GLUT4결합활성급GLUT4기인표체량적조절작용,이료해운동제고당뇨병대서GLUT4기인표체적가능궤제.장건강SD대서100지,40지작위정상대조,기여60지건립당뇨병대서모형,건모성공35지.정상대조화당뇨병대서재안체중각자수궤분위5조:안정대조조、일차성운동조、일차성운동+KN93조、내력훈련조、내력훈련+KN93조,공10조.포태속도20 m/min,운동시간1 h.일차성운동조대서1차운동후3 h취재.내력훈련조채용동양포태속도화운동시간,훈련1주,병우최후1차훈련후12 h취재.결과득도,당뇨병대서내력훈련조,골격기세포핵내MEF2화GLUT4적결합활성급골격기 GLUT4기인표체량수연균현저저우정상대조대서내력훈련조,단여당뇨병대서안정대조조상비균현저승고;내력훈련+KN93조,골격기세포핵내MEF2화GLUT4적결합활성여안정대조조상비차이몰유현저성,단골격기 GLUT4기인표체량여안정대조조상비각현저승고.결과현시:수연운동통과격활당뇨병대서골격기CAMK II이제고기골격기세포핵내MEF2화GLUT4적결합활성,수연MEF2화GLUT4적결합활성삼여조해료정상대서골격기GLUT4기인표체,단병불시영향당뇨병대서골격기GLUT4기인표체적유일인소.
In order to study the role played by CaMKII activated by exercising in adjusting the activity of combination of MEF2 and GLUT4 and the level of expression of gene GLUT4 in skeletal muscle of diabetic rats, so as to probe into any possible mechanism of exercising enhancing the expression of gene GLUT4 of diabetic rats, the author divided 100 healthy SD rats into a normal control group which contains 40 rats and a diabetic rat model establishment group which contains remaining 60 rats, 35 of which were successfully model established. Normal control rats and diabetic rats were randomly divided into 5 groups respectively by weight:a calm control group, a one-time exercising group, a one-time exercising+KN93 group, an endurance training group, an endurance+KN93 group;there were 10 groups totally. The treadmill speed was 20m/min;the exercising time was 1 h. The rats in the one-time exercising groups were sample taken 3h after one-time exercising. The rats in the endurance training groups were trained for 1 week using the same treadmill speed and exercising time, and sample taken 12 h after the last training. The author revealed the following findings:the activity of combination of MEF2 and GLUT4 inside skeletal muscle cells and the level of expression of gene GLUT4 in skeletal muscle of the rats in the diabetic rat endurance training group were all significantly lower than those of the rats in the normal control rat endurance training group, but significantly higher than those of the rats in the rats in the diabetic rat calm control group;the activity of combination of MEF2 and GLUT4 inside skeletal muscle cells of the rats in the endurance training+KN93 group was not significantly different from that of the rats in the calm con-trol group, but the level of expression of gene GLUT 4 in skeletal muscle of the rats in the endurance training+KN93 group was significantly higher than that of the rats in the calm control group. The findings indicate the followings:al-though exercising enhances the activity of combination of MEF2 and GLUT4 inside skeletal muscle by activating skeletal muscle CaMK II of diabetic rats, and the activity of combination of MEF2 and GLUT4 participates in regulat-ing the expression of gene GLUT4 in skeletal muscle of normal rats, it is not the only factor that affects the expression of gene GLUT4 in skeletal muscle of diabetic rats.