体育学刊
體育學刊
체육학간
JOURNAL OF PHYSICAL EDUCATION
2013年
2期
130-134
,共5页
运动生物化学%2型糖尿病%运动训练%心肌纤维化%氧化应激%大鼠
運動生物化學%2型糖尿病%運動訓練%心肌纖維化%氧化應激%大鼠
운동생물화학%2형당뇨병%운동훈련%심기섬유화%양화응격%대서
sports biochemistry%type 2 diabetes%exercising%myocardial fibrosis%oxidative stress%rats
通过高脂喂养加腹腔注射小剂量链脲佐菌素(STZ)构建2型糖尿病大鼠模型,探讨运动训练对2型糖尿病大鼠心肌纤维化的改善作用.结果发现:与正常对照组相比,糖尿病对照组血液GLU、GHb、GSP和INS水平及心肌AGEs、Hyp、CVF、GSSG/GSH、MDA和NF-κB P65蛋白表达均显著升高(P<0.05或P<0.01);与糖尿病对照组相比,糖尿病运动组(DE)的血液GLU、GHb和GSP水平及心肌AGEs、Hyp、CVF、GSSG/GSH、MDA和NF-κB P65蛋白表达均显著降低(P<0.05或P<0.01).结果表明:本实验所复制的2型糖尿病大鼠模型存在心肌纤维化的现象,运动可改善2型糖尿病大鼠空腹血糖,降低心肌组织AGEs含量、氧化应激和NF-κB P65蛋白表达水平,对糖尿病大鼠心肌纤维化起到改善作用,AGEs/氧化应激/NF-κB P65可能为运动改善2型糖尿病大鼠心肌纤维化的重要通路.
通過高脂餵養加腹腔註射小劑量鏈脲佐菌素(STZ)構建2型糖尿病大鼠模型,探討運動訓練對2型糖尿病大鼠心肌纖維化的改善作用.結果髮現:與正常對照組相比,糖尿病對照組血液GLU、GHb、GSP和INS水平及心肌AGEs、Hyp、CVF、GSSG/GSH、MDA和NF-κB P65蛋白錶達均顯著升高(P<0.05或P<0.01);與糖尿病對照組相比,糖尿病運動組(DE)的血液GLU、GHb和GSP水平及心肌AGEs、Hyp、CVF、GSSG/GSH、MDA和NF-κB P65蛋白錶達均顯著降低(P<0.05或P<0.01).結果錶明:本實驗所複製的2型糖尿病大鼠模型存在心肌纖維化的現象,運動可改善2型糖尿病大鼠空腹血糖,降低心肌組織AGEs含量、氧化應激和NF-κB P65蛋白錶達水平,對糖尿病大鼠心肌纖維化起到改善作用,AGEs/氧化應激/NF-κB P65可能為運動改善2型糖尿病大鼠心肌纖維化的重要通路.
통과고지위양가복강주사소제량련뇨좌균소(STZ)구건2형당뇨병대서모형,탐토운동훈련대2형당뇨병대서심기섬유화적개선작용.결과발현:여정상대조조상비,당뇨병대조조혈액GLU、GHb、GSP화INS수평급심기AGEs、Hyp、CVF、GSSG/GSH、MDA화NF-κB P65단백표체균현저승고(P<0.05혹P<0.01);여당뇨병대조조상비,당뇨병운동조(DE)적혈액GLU、GHb화GSP수평급심기AGEs、Hyp、CVF、GSSG/GSH、MDA화NF-κB P65단백표체균현저강저(P<0.05혹P<0.01).결과표명:본실험소복제적2형당뇨병대서모형존재심기섬유화적현상,운동가개선2형당뇨병대서공복혈당,강저심기조직AGEs함량、양화응격화NF-κB P65단백표체수평,대당뇨병대서심기섬유화기도개선작용,AGEs/양화응격/NF-κB P65가능위운동개선2형당뇨병대서심기섬유화적중요통로.
The authors established a type 2 diabetic rat model by means of high fat feeding plus intraperitoneal injec-tion of a small dose of streptozotocin (STZ), probed into the role of exercising in improving the myocardial fibrosis of type 2 diabetic rats, and revealed the following findings:as compared with those of the rats in the normal control group, the blood GLU, GHb, GSP and INS level as well as myocardium AGEs, Hyp, CVF, GSSG/GSH, MDA and NF-κB P65 protein expression of the rats in the diabetic control group increased significantly (P<0.05 or P<0.01);as compared with those of the rats in the diabetic control group, the blood GLU, GHb and GSP level as well as myocardium AGEs, Hyp, CVF, GSSG/GSH, MDA and NF-κB P65 protein expression of the rats in the diabetic exercising group decreased significantly (P<0.05 or P<0.01). The said findings indicated the followings:the type 2 diabetic rat model duplicated by this experiment showed such a sign as myocardial fibrosis;exercising could improve the fasting blood glucose of type 2 diabetic rats, lower myocardial tissue’s AGEs content, oxidative stress and the level of NF-κB P65 protein expression, play a role in improving the myocardial fibrosis of type 2 diabetic rats;AGEs or oxidative stress or NF-κB P65 might be an important channel for exercising to improve the myocardial fibrosis of type 2 diabetic rats.
