CAJ | 학술논문

研究6周递增负荷运动对小肠集合淋巴结(PP结)线粒体膜电位(ΔΨm)、BaxmRNA、Bcl-2mRNA和细胞色素C(Cyt C)的影响,探讨PP结淋巴细胞线粒体凋亡途径指标的变化.将64只8周龄雄性SD大鼠随机分为运动组和对照组,运动组进行6周的递增负荷跑台训练,分别于第0、2、4、6周末,利用FQ-RT-PCR技术测定Bax和Bcl-2mRNA的表达水平;利用JC-1染色流式细胞术检测PP结淋巴细胞的平均J-aggregate( FL2)和J-monomer( FL1)的含量,并计算ΔΨm( FL2/FL1);用ELISA法测定Cyt C的浓度.结果显示:(1)6周递增负荷运动过程中,在第2、4、6周,PP结淋巴细胞的ΔΨm显著低于0周,第4周的ΔΨm最低;(2)与0周相比,第4、6周PP结BaxmRNA表达明显增加,Bcl-2mRNA表达降低,Bcl-2/Bax值明显降低;(3)与0周相比,第2、4、6周PP结Cyt C浓度明显增加.结果表明递增负荷运动可能是通过影响线粒体途径从而影响PP结淋巴细胞凋亡的.
연구6주체증부하운동대소장집합림파결(PP결)선립체막전위(ΔΨm)、BaxmRNA、Bcl-2mRNA화세포색소C(Cyt C)적영향,탐토PP결림파세포선립체조망도경지표적변화.장64지8주령웅성SD대서수궤분위운동조화대조조,운동조진행6주적체증부하포태훈련,분별우제0、2、4、6주말,이용FQ-RT-PCR기술측정Bax화Bcl-2mRNA적표체수평;이용JC-1염색류식세포술검측PP결림파세포적평균J-aggregate( FL2)화J-monomer( FL1)적함량,병계산ΔΨm( FL2/FL1);용ELISA법측정Cyt C적농도.결과현시:(1)6주체증부하운동과정중,재제2、4、6주,PP결림파세포적ΔΨm현저저우0주,제4주적ΔΨm최저;(2)여0주상비,제4、6주PP결BaxmRNA표체명현증가,Bcl-2mRNA표체강저,Bcl-2/Bax치명현강저;(3)여0주상비,제2、4、6주PP결Cyt C농도명현증가.결과표명체증부하운동가능시통과영향선립체도경종이영향PP결림파세포조망적.
The authors studied the effects of 6-week load gradually increased exercising on the membrane potential (ΔΨm), BaxmRNA, Bcl-2mRNA and Cytochrome C (Cyt C) of mitochondria in Peyer’s patches, and probed into the changing of such an index as mitochondrial apoptosis pathway in Peyer’s patches. The authors divided 64 8-week old male SD rats randomly into an exercising group and a control group, let the rats in the exercising group do a load gradually in-creased exercise on a treadmill for 6 weeks, measured the levels of Bax and Bcl-2mRNA expression by utilizing the FQ-RT-PCR technology and the average contents of J-aggregate ( FL2 ) and J-monomer ( FL1) of lymphocyte in Peyer’s patches by utilizing JC-1 staining flow cytometry at the end of weeks 0, 2 4 and 6 respectively, calculated ΔΨm ( FL2/FL1), and measured the concentration of Cyt C by apply the ELISA method. The authors revealed the following findings:1) during 6-week load gradually increased exercising, the ΔΨms of lymphocytes in Peyer’s patches calculated in weeks 2, 4 and 6 were significantly lower than that calculated in week 0, the ΔΨm calculated in week 4 was the lowest;2) as compared with those measured in week 0, measured in weeks 4 and 6, the levels of BaxmRNA expression in Peyer’s patches increased significantly, the levels of Bcl-2mRNA expression decreased, and the ratio of Bcl-2 to Bax decreased significantly;3) as compared with those measured in week 0, the concentrations of Cyt C in Peyer’s patches measured in weeks 2, 4 and 6 increased significantly. The said findings indicated that load gradually increased exercising might affect the apoptosis of lymphocytes in Peyer’s patches by affecting the mitochondrial pathway.