CAJ | 학술논문

运用显微介导远缘杂交技术,将未经遗传修饰的中国对虾(Fenneropenaeus chinensis)总DNA直接导入受体鲤(Cyprinus carpio L.)内,以期通过这种基因组水平的远缘杂交技术改善受体鲤的品质.创建的远缘基因鲤提高了鲤的品质,丰富了鲤的遗传基础.本研究对48尾显微介导远缘基因鲤亲鱼进行标记取样,通过20个具有多态的鲤微卫星引物分析,选出在中度遗传距离(0.5~0.6)的亲本进行繁殖,从子代群体中选出品质性状优良的3个家系为研究对象.利用微卫星标记,分别对3个家系进行了遗传背景分析.结果表明,3个家系的平均有效等位基因数(Ne)分别为2.1535、2.3840和2.4110;平均观测杂合度(Ho)分别为0.4825、0.4300和0.4450;平均期望杂合度(He)分别为0.5195、0.5720和0.5700;平均多态信息含量(PIC)分别为0.4300、0.4715和0.4945.其遗传多样性处于中度多态(0.25<PIC<0.5)偏高水平.3个家系的基因分化系数(GST)为0.074,家系之间属于中度分化水平.
운용현미개도원연잡교기술,장미경유전수식적중국대하(Fenneropenaeus chinensis)총DNA직접도입수체리(Cyprinus carpio L.)내,이기통과저충기인조수평적원연잡교기술개선수체리적품질.창건적원연기인리제고료리적품질,봉부료리적유전기출.본연구대48미현미개도원연기인리친어진행표기취양,통과20개구유다태적리미위성인물분석,선출재중도유전거리(0.5~0.6)적친본진행번식,종자대군체중선출품질성상우량적3개가계위연구대상.이용미위성표기,분별대3개가계진행료유전배경분석.결과표명,3개가계적평균유효등위기인수(Ne)분별위2.1535、2.3840화2.4110;평균관측잡합도(Ho)분별위0.4825、0.4300화0.4450;평균기망잡합도(He)분별위0.5195、0.5720화0.5700;평균다태신식함량(PIC)분별위0.4300、0.4715화0.4945.기유전다양성처우중도다태(0.25<PIC<0.5)편고수평.3개가계적기인분화계수(GST)위0.074,가계지간속우중도분화수평.
In this study, genome DNA from shrimp (Fenneropenaeus chinensis) without genetic modification was transferred into common carp (Cyprinus carpio L.) using micro-injected distant-hybridization, expecting to improve quaility of common carp at the genome level, and the muscle quality and and heredity was actually im-proved. DNA from 48 micro-injected exogenous gene carp were extracted and analyzed by 20 microsatellite markers. Individuals with moderate genetic distance(0.5-0.6) were selected as parents for breeding, and 3 superior families were obtained from the filial generation and the genetic background of the three families were analyzed using microsatellite markers. The results indicate that the average effective number of allels (Ne) was 2.153 5, 2.384 0 and 2.411 0, respectively;average observed heterozygosity (Ho) was 0.482 5, 0.430 0 and 0.445 0, respec-tively;average expected heterozygosity (He) was 0.5195, 0.572 and 0.57, respectively;and the average polymor-phism information content (PIC) was 0.430 0, 0.471 5 and 0.494 5, respectively. It suggested that the genetic di-versities were at a middle level (0.25<PIC<0.5), close to high. Gene differentiation coefficient of the three fami-lies was 0.074, at a moderate differentiation level.