CAJ | 학술논문

粳稻品种“嘉花1号”经60Coγ射线辐照后,在其后代中筛选到一个黄叶的突变体(yl6),经过表型分析,发现该突变体幼苗期不论在低温(20℃)还是在高温(32℃)培养条件下,与野生型相比叶色均呈现出淡黄色,表明其为一温度不敏感突变体.光合色素含量测定结果显示,yl6突变体的黄叶突变性状主要是由叶绿素含量下降所导致.电镜结果显示,yl6突变体内叶绿素合成受阻且叶绿体的正常发育受到影响.遗传分析表明,该突变性状受一对隐性核基因(yl6)所控制.利用该突变体与籼稻“培矮64S”杂交产生的F2、F3群体中分离出的608个突变体型单株作为定位群体,结合SSR和CAPS分子标记将yl6基因定位在水稻第6染色体短臂上的CAPS1和RM2353分子标记之间,其物理距离约为271 kb,目前该区域内没有发现与水稻叶绿素合成/叶绿体发育相关已知功能基因.本研究结果可为yl6基因的克隆和功能分析奠定了基础.
갱도품충“가화1호”경60Coγ사선복조후,재기후대중사선도일개황협적돌변체(yl6),경과표형분석,발현해돌변체유묘기불론재저온(20℃)환시재고온(32℃)배양조건하,여야생형상비협색균정현출담황색,표명기위일온도불민감돌변체.광합색소함량측정결과현시,yl6돌변체적황협돌변성상주요시유협록소함량하강소도치.전경결과현시,yl6돌변체내협록소합성수조차협록체적정상발육수도영향.유전분석표명,해돌변성상수일대은성핵기인(yl6)소공제.이용해돌변체여선도“배왜64S”잡교산생적F2、F3군체중분리출적608개돌변체형단주작위정위군체,결합SSR화CAPS분자표기장yl6기인정위재수도제6염색체단비상적CAPS1화RM2353분자표기지간,기물리거리약위271 kb,목전해구역내몰유발현여수도협록소합성/협록체발육상관이지공능기인.본연구결과가위yl6기인적극륭화공능분석전정료기출.
@@@@Through observation of phenotypic trait of the progeny of a japonica rice variety "Jiahua 1" treated with 60Coγ-radiation, a yellow-leaf mutant (yl6) in rice (Oryza sativa L.) was selected. During the seedling stage, the mutant exhibited light-yellow-leaf phenotype both at the low (20℃) and high temperature (32℃). These results indicated that yl6 is a themo-insensitive mutant. The light-yellow-leaf phenotype of yl6 was mainly caused by the declining of chlorophyll. Electron microscope observation indicated that the biosynthesis of chloroplastwas hindered and development of chloroplast was affected in yl6 mutant. Genetic analysis showed that the phenotype of yl6 mutant was controlled by a single recessive nuclear gene that named yl6. By using SSR and CAPS molecular markers and 608 individuals of F2 and F3 generation with mutant phenotype derived from the cross of yl6 and the indica variety Peiai 64S, the yl6 was mapped between the molecular markers CAPS1 and RM2353 with 271 kb of physical distance on the short arm of chromosome 6 in rice. There was no gene conferring chlorophyll synthesis or chloroplast development in rice ever being reported in this genomic region. The findings in this study would provide a foundation for the cloning and functional analysis of the yl6 gene in the future.