CAJ | 학술논문

番茄黄化曲叶病毒病(tomato yellow leaf curl virus disease, TYLCD)是番茄生产上最严重的病害之一.为了更有效地分析其致病机理,本研究根据已知的番茄黄化曲叶病毒(tomato yellow leaf curl virus, TYLCV)基因组序列设计引物,通过PCR扩增技术,分离浙江省TYLCV的全长序列,分析其结构特征,序列同源性及系统发育关系.结果表明:病毒基因全长为2781个核苷酸,共编码6个开放阅读框(AV1, AV2, AC3, AC2, AC1, AC4),即TYLCV的典型结构;核苷酸序列比对分析发现该病毒分离物与美国的TYLCV同源性最高(99.6%);6个编码区分析显示除云南外,AV2与国内其他地区同源性均高达100%;系统发育关系分析表明病毒分离物与江苏、北京、美国和墨西哥的TYLCV划分为一类,亲缘关系最近.这些结果为今后揭示TYLCV的致病机理奠定基础,并为番茄黄化曲叶病毒病的诊断和防治提供科学依据.
번가황화곡협병독병(tomato yellow leaf curl virus disease, TYLCD)시번가생산상최엄중적병해지일.위료경유효지분석기치병궤리,본연구근거이지적번가황화곡협병독(tomato yellow leaf curl virus, TYLCV)기인조서렬설계인물,통과PCR확증기술,분리절강성TYLCV적전장서렬,분석기결구특정,서렬동원성급계통발육관계.결과표명:병독기인전장위2781개핵감산,공편마6개개방열독광(AV1, AV2, AC3, AC2, AC1, AC4),즉TYLCV적전형결구;핵감산서렬비대분석발현해병독분리물여미국적TYLCV동원성최고(99.6%);6개편마구분석현시제운남외,AV2여국내기타지구동원성균고체100%;계통발육관계분석표명병독분리물여강소、북경、미국화묵서가적TYLCV화분위일류,친연관계최근.저사결과위금후게시TYLCV적치병궤리전정기출,병위번가황화곡협병독병적진단화방치제공과학의거.
@@@@Tomato Yellow Leaf Curl Virus Disease (TYLCD) is one of the most serious diseases in tomato production. To analyze the pathogenic mechanism, a full-length sequence of virus isolate was cloned by PCR amplification based on a pair of specific primers designed according to known genome sequence of TYLCV. Then, structure feature, homology comparison and phylogenetic analysis of this virus isolate were conducted. The results indicated that total length of the virus isolate was 2 781 nucleotides in length encoding six open reading frames (ORFs), i.e. AV2, AV1, AC3, AC2, AC1, AC4, that are the typical structure of TYLCV. Nucleotide sequence analysis showed that the virus isolates in Zhejiang province of East China had the highest homology (99.6%) with that of TYLCV in USA. ORFs analysis suggested that AV2 should be 100% homology with other province in China except Yunnan province of Southwest China. Phylogenetic analysis exhibited that the virus isolate was assigned to the group of Jiangsu, Beijing, USA, and Mexico, indicating the closest genetic relationship. The above results not only contributed to reveal the pathogenic mechanisms of TYLCV, but also laid the scientific foundation for the diagnosis and prevention of TYLCD.