CAJ | 학술논문

几丁质酶是一种重要的糖苷酶,在植物抗生物及非生物胁迫的过程中发挥了重要的作用.根据序列差异及所含结构域的不同,杨树中的几丁质酶可以分为5类.其中关于第Ⅴ类几丁质酶的研究最少.本研究在加拿大杨中克隆到了一个第Ⅴ类几丁质酶基因PcCHI3.序列分析的结果显示,该基因cDNA全长1303 bp,含完整的开放阅读框,可编码366个氨基酸.预测蛋白质分子质量为39.53 kD.理论等电点(pI)为5.49.PcCHI3与苦瓜、烟草、梨的第Ⅴ类几丁质酶在系统进化树上同属一个分支.对PcCHI3在逆境胁迫及病原真菌诱导下的表达模式进行分析后发现,该基因可以被杨树病原真菌杨生褐盘二孢菌强烈诱导(可达对照的83倍),在干旱胁迫及盐胁迫的条件下表达量也出现了较大的上调(为对照的4~5倍).研究结果为杨树几丁质酶功能的系统进化及林木抗性育种研究建立了一定基础.
궤정질매시일충중요적당감매,재식물항생물급비생물협박적과정중발휘료중요적작용.근거서렬차이급소함결구역적불동,양수중적궤정질매가이분위5류.기중관우제Ⅴ류궤정질매적연구최소.본연구재가나대양중극륭도료일개제Ⅴ류궤정질매기인PcCHI3.서렬분석적결과현시,해기인cDNA전장1303 bp,함완정적개방열독광,가편마366개안기산.예측단백질분자질량위39.53 kD.이론등전점(pI)위5.49.PcCHI3여고과、연초、리적제Ⅴ류궤정질매재계통진화수상동속일개분지.대PcCHI3재역경협박급병원진균유도하적표체모식진행분석후발현,해기인가이피양수병원진균양생갈반이포균강렬유도(가체대조적83배),재간한협박급염협박적조건하표체량야출현료교대적상조(위대조적4~5배).연구결과위양수궤정질매공능적계통진화급림목항성육충연구건립료일정기출.
@@@@As one of the most important Glycoside hydrolases, Chitinases are involved in multiple stress responses. Based on amino acid sequence similarities, all plant chitinases can be divided into 5 classes. Among them, class Ⅴ was relative less studied yet. A classⅤ chtinase gene from Populus was cloned to uncover its potential roles. We named this gene PcCHI3. The full-length cDNA of this gene is 1 303 bp. It encodes 366 amino acid residues with a molecular mass around 39.53 kD and a theoretical isoelectric point (pI) of 5.49. Phylogenetic analysis showed that PcCHI3 belonged to a clade with other classⅤ chtinase genes from Momordica charantia, Nicotiana tabacum and pyrus pyrifolia. Analysis on the expression patterns of PcCHI3 revealed that not only fungal pathogen infection, but also drought and salt stresses could induce strong accumulation of this gene. It provides evidence that PcCHI3 play a crucial role in resistance against both biotic and abiotic stresses. This study will shed some light on further investigation of Populus chitinase genes and lay a base for the stress resistance breeding of woody trees.