CAJ | 학술논문

为了去除体系优化评分的人为因素干扰,尝试用Photoshop软件代替人工评分.实验采用L16(45)正交表进行4因素4水平实验,得出电泳结果,用Photoshop软件对16个处理进行评分,得出在月季SSR 10μL反应体系中最适浓度为Taq酶0.75 U,DNTP 0.3 mmol/L,Mg2+1.0 mmol/L,引物0.6μmol/L.最后21个月季品种进行验证试验,证明该体系配比合理,说明Photoshop评分是一种有效的评分方法,可以应用于体系优化评分.
위료거제체계우화평분적인위인소간우,상시용Photoshop연건대체인공평분.실험채용L16(45)정교표진행4인소4수평실험,득출전영결과,용Photoshop연건대16개처리진행평분,득출재월계SSR 10μL반응체계중최괄농도위Taq매0.75 U,DNTP 0.3 mmol/L,Mg2+1.0 mmol/L,인물0.6μmol/L.최후21개월계품충진행험증시험,증명해체계배비합리,설명Photoshop평분시일충유효적평분방법,가이응용우체계우화평분.
@@@@For excluding the interference of human factors in optimization of SSR system, we attempted to use Photoshop instead of artificial score. The experiment was conducted four factors and four levels, then using Photoshop to analysis the 16 treatments. The results showed that rTaq DNA polymerase 0.75 U, dNTP 0.3 mmol/L,Mg2+ 1.0 mmol/L, primer 0.6 μmol/L is the optimal concentration of rose SSR reaction system. At last 21 Rose varieties were chose to verification the test, to prove that the system is in a reasonable ratio, so that Photoshop is an effective scoring method can be applied to system optimization score.