CAJ | 학술논문

采用通用引物对山东长岛、文登、日照、广东湛江和海南三亚等5个地理群体栉江珧(Atrina pectinta)16S rRNA序列进行扩增、测序分析,得到59条441bp的核苷酸序列.其中T、C、A、G和A+T的平均含量分别为29.91%、17.41%、25.74%、26.94%及55.65%, AT含量高于GC含量,共检测到了9个单倍型和26个核苷酸多态位点.群体多样性分析表明,文登群体具有较高的遗传多样性水平.AMOVA 分析表明,五群体间总遗传分化系数Fst =0.5007(P<0.001),群体间遗传分化略大于群体内、群体间存在较高的遗传分化.基于群体间遗传距离构建NJ和UPGMA分子进化树,5个地理群体的栉江珧聚为两个支,长岛、文登、日照群体聚为一支,海南和湛江群体聚为另一支.
채용통용인물대산동장도、문등、일조、엄동담강화해남삼아등5개지리군체즐강요(Atrina pectinta)16S rRNA서렬진행확증、측서분석,득도59조441bp적핵감산서렬.기중T、C、A、G화A+T적평균함량분별위29.91%、17.41%、25.74%、26.94%급55.65%, AT함량고우GC함량,공검측도료9개단배형화26개핵감산다태위점.군체다양성분석표명,문등군체구유교고적유전다양성수평.AMOVA 분석표명,오군체간총유전분화계수Fst =0.5007(P<0.001),군체간유전분화략대우군체내、군체간존재교고적유전분화.기우군체간유전거리구건NJ화UPGMA분자진화수,5개지리군체적즐강요취위량개지,장도、문등、일조군체취위일지,해남화담강군체취위령일지.
Mitochondrial 16S rRNA gene fragments of Atrina pectinta sampled from five locations (Changdao, Wendeng, Rizhao, Zhanjiang and Sanya) were amplified with universal primer;the PCR products were sequenced, and 441bp nucleotide sequences were obtained. The T, C, A, G and A+T contents in this fragment were 29.91%, 17.41%, 25.74%, 26.94%and 55.65%, respectively. The AT content was higher than the GC content. The diversity of the population analysis showed that the population of Wendeng had higher level of genetic diversity. Nine hap-lotypes were defined among five populations, which were from 26 segregating sites. The fixation indices (Fst) ana-lyzed by AMOVA totaled to Fst=0.5007 (P<0.001) showing that the genetic fixation inter-populations was a little larger than that intra-populations and indicated significant genetic fixation among the five populations. The Neighbor-joining (NJ) phylogenetic tree and UPGMA phylogenetic tree were made from the five populations of A. pectinta. The result showed that the population of WD, CD and RZ might have originated from one clade, while populations of HN and ZJ from another.