CAJ | 학술논문

以副溶血性弧菌 VP-X-3为试验对象,利用气相色谱技术分析了其细胞脂肪酸的组成和含量,以色谱峰的数量、色谱峰高及脂肪酸含量作为评价指标,探讨了不同的皂化和甲酯化条件对细菌细胞脂肪酸测定结果的影响.结果表明:皂化和甲酯化条件都对细胞脂肪酸的组成和含量产生一定的影响.较低温度皂化处理利于增加色谱峰数量和色谱峰高.甲酯化温度过高容易破坏脂肪酸,尤其是长链脂肪酸和不饱和脂肪酸的结构.高温甲酯化条件下,时间越长,甲酯化效果越差.试验得到较为理想的气相色谱前处理条件:2 mol/L NaOH-甲醇溶液2 mL,70℃水浴10 min;10%(V/V)H2SO4-甲醇溶液2 mL,70℃水浴15 min.此条件下的色谱峰的数量多达29种,色谱峰高明显高于其他处理条件下的峰高.
이부용혈성호균 VP-X-3위시험대상,이용기상색보기술분석료기세포지방산적조성화함량,이색보봉적수량、색보봉고급지방산함량작위평개지표,탐토료불동적조화화갑지화조건대세균세포지방산측정결과적영향.결과표명:조화화갑지화조건도대세포지방산적조성화함양산생일정적영향.교저온도조화처리리우증가색보봉수량화색보봉고.갑지화온도과고용역파배지방산,우기시장련지방산화불포화지방산적결구.고온갑지화조건하,시간월장,갑지화효과월차.시험득도교위이상적기상색보전처리조건:2 mol/L NaOH-갑순용액2 mL,70℃수욕10 min;10%(V/V)H2SO4-갑순용액2 mL,70℃수욕15 min.차조건하적색보봉적수량다체29충,색보봉고명현고우기타처리조건하적봉고.
The composition and percentage of fatty acids in Vibrio parahaemolyticus VP-X-3 were analyzed by gas chromatography. With quantity, peak height and percentage of fatty acids showed in the chromatography as the standard, the saponification and methyl esterification conditions were also investigated to give an optimum pre-treatment method. The results indicated that both saponification and methyl esterification influenced fatty acids composition and percentage. The peak quantity and height were increased by lowering saponification temperature. Too high methyl esterification temperature would easily damage fatty acids, especially the structure of long-chain fatty acids and unsaturated fatty acids. The esterification effect would be worse with longer esterification time at higher esterification temperature. The optimum gas chromatography pretreatments were as follows:saponification, 2 mL 2 mol/L NaOH-methanol, water bath at 70 for 10 min;methyl esterification, 2 mL 10%H℃ 2SO4-methanol, water bath at 70 for 15 min. On the chromatogram of GC, the quantity of chromatographic peak was as many as℃29, and the peak height was distinctly higher than those with other different pretreatments.