安徽农业大学学报
安徽農業大學學報
안휘농업대학학보
JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY
2013年
1期
120-123
,共4页
抗汞菌株%API20E鉴定%质粒%汞去除
抗汞菌株%API20E鑒定%質粒%汞去除
항홍균주%API20E감정%질립%홍거제
mercury-resistance strain%API20E identification%plasmid%Hg2+removal
利用 API20E 细菌鉴定系统对筛选获得的抗汞菌株 PII 进行鉴定,并采用高温-SDS 对所获抗汞菌株PII进行质粒消除,研究其对PII菌株的去汞特性的影响.结果表明,抗汞菌株PII生化谱为2227004,经API20E生化项分析鉴定系统查询,为恶臭假单胞菌(Pseudomonas putida);抗汞菌株PII含有质粒,其质粒片段在4 kb~5 kb左右,利用高温-SDS法质粒消除率达到62.5%.质粒消除试验表明,抗汞菌株PII的汞去除能力与质粒相关.
利用 API20E 細菌鑒定繫統對篩選穫得的抗汞菌株 PII 進行鑒定,併採用高溫-SDS 對所穫抗汞菌株PII進行質粒消除,研究其對PII菌株的去汞特性的影響.結果錶明,抗汞菌株PII生化譜為2227004,經API20E生化項分析鑒定繫統查詢,為噁臭假單胞菌(Pseudomonas putida);抗汞菌株PII含有質粒,其質粒片段在4 kb~5 kb左右,利用高溫-SDS法質粒消除率達到62.5%.質粒消除試驗錶明,抗汞菌株PII的汞去除能力與質粒相關.
이용 API20E 세균감정계통대사선획득적항홍균주 PII 진행감정,병채용고온-SDS 대소획항홍균주PII진행질립소제,연구기대PII균주적거홍특성적영향.결과표명,항홍균주PII생화보위2227004,경API20E생화항분석감정계통사순,위악취가단포균(Pseudomonas putida);항홍균주PII함유질립,기질립편단재4 kb~5 kb좌우,이용고온-SDS법질립소제솔체도62.5%.질립소제시험표명,항홍균주PII적홍거제능력여질립상관.
API 20E was used to identify isolated strain PII, and Hg2+removal of PII was tested after plasmid elimination by high temperature and SDS. The results showed that the biochemical spectrum of PII was 2227004, and PII was identified as Pseudomonas putida by API20E;PII had plasmids, and its plasmid clips was 4 kb-5 kb;plasmid elimination rate by high temperature and SDS was 62.5%;the plasmid elimination test showed that the gene which decided strain PII to resist or remove mercury was on the plasmid.
