安徽农业大学学报
安徽農業大學學報
안휘농업대학학보
JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY
2013年
1期
144-148
,共5页
张磊%鲍先巡%刘明辉%韩冷%中垣雅雄
張磊%鮑先巡%劉明輝%韓冷%中垣雅雄
장뢰%포선순%류명휘%한랭%중원아웅
傅里叶变换拉曼光谱%牵引丝%分子结构
傅裏葉變換拉曼光譜%牽引絲%分子結構
부리협변환랍만광보%견인사%분자결구
Raman spectroscopy%dragline silk fiber%molecular structure
利用傅里叶变换拉曼光谱技术对不同吐丝环境下获得的棒络新妇蜘蛛牵引丝(自由爬行分泌的牵引丝,垂直下落时分泌的牵引丝,在20 mm·s-1的速度下人工强制抽取的牵引丝)纤维大分子结构进行了分析,对特征谱带的分析结果表明,蜘蛛牵引丝蛋白中包含了β-折叠,β-转角,α-螺旋以及无规卷曲结构;酰胺I带中,不同的方式获得的牵引丝中所含的4种二级结构的比例不同.其中人工强制抽取获得的牵引丝中包含较多的β-折叠结构,α-螺旋含量最少,说明在不同环境条件下,蜘蛛分泌牵引丝的结构会发生变化,这一改变可能会导致丝纤维的力学特性的改变.
利用傅裏葉變換拉曼光譜技術對不同吐絲環境下穫得的棒絡新婦蜘蛛牽引絲(自由爬行分泌的牽引絲,垂直下落時分泌的牽引絲,在20 mm·s-1的速度下人工彊製抽取的牽引絲)纖維大分子結構進行瞭分析,對特徵譜帶的分析結果錶明,蜘蛛牽引絲蛋白中包含瞭β-摺疊,β-轉角,α-螺鏇以及無規捲麯結構;酰胺I帶中,不同的方式穫得的牽引絲中所含的4種二級結構的比例不同.其中人工彊製抽取穫得的牽引絲中包含較多的β-摺疊結構,α-螺鏇含量最少,說明在不同環境條件下,蜘蛛分泌牽引絲的結構會髮生變化,這一改變可能會導緻絲纖維的力學特性的改變.
이용부리협변환랍만광보기술대불동토사배경하획득적봉락신부지주견인사(자유파행분비적견인사,수직하락시분비적견인사,재20 mm·s-1적속도하인공강제추취적견인사)섬유대분자결구진행료분석,대특정보대적분석결과표명,지주견인사단백중포함료β-절첩,β-전각,α-라선이급무규권곡결구;선알I대중,불동적방식획득적견인사중소함적4충이급결구적비례불동.기중인공강제추취획득적견인사중포함교다적β-절첩결구,α-라선함량최소,설명재불동배경조건하,지주분비견인사적결구회발생변화,저일개변가능회도치사섬유적역학특성적개변.
Raman spectra of Nephia clavata spider dragline silk fibers were obtained by three different spin-ning methods:crawl spinning, drop spinning and artificial reeling at 20 mm/s. Based on a rational decomposition of the amide I spectra, the structural conformation of dragline silk proteins and the proportion of those secondary structure were determined. This analysis shows that spider dragline silk containsβ-sheet,β-turn,α-helix and un-ordered structure elements;however, the proportions in which these structures appear changes with the spinning method. Theβ-sheet content of silk fiber obtained by artificial reeling at 20 mm/s is higher than that of spider spun fibers, indicating that some of the α-helix and other structures are transformed into β-sheet during the spider’s spinning process.