CAJ | 학술논문

肥大细胞受到抗原刺激后释放组胺是过敏反应的重要过程,对组胺释放的抑制能力是评价抗过敏药物的重要因素.选取多种传统中医记载具有抗过敏作用的中草药,检测了其在体外对肥大细胞脱颗粒并释放组胺的抑制效果.在具有明显效果的中草药中,土茯苓与芍药对于抑制肥大细胞脱颗粒最为突出, IC50分别为62.5μg/mL和113.8μg/mL.土茯苓的突出药效(药物浓度为500μg/mL时抑制率达到95.63%)未曾被报道,具有研究与开发价值.对其机理的初步研究表明蛋白激酶A可能是土茯苓抑制肥大细胞脱颗粒的重要靶点.
비대세포수도항원자격후석방조알시과민반응적중요과정,대조알석방적억제능력시평개항과민약물적중요인소.선취다충전통중의기재구유항과민작용적중초약,검측료기재체외대비대세포탈과립병석방조알적억제효과.재구유명현효과적중초약중,토복령여작약대우억제비대세포탈과립최위돌출, IC50분별위62.5μg/mL화113.8μg/mL.토복령적돌출약효(약물농도위500μg/mL시억제솔체도95.63%)미증피보도,구유연구여개발개치.대기궤리적초보연구표명단백격매A가능시토복령억제비대세포탈과립적중요파점.
Mast cell is considered to be a key actor for the allergic inflammation. Upon presentation of the antigen they could release histamine. The inhibition of histamine releasing is usually used as major element to evaluate the anti-allergy activity of drugs. Diverse herbs which released allergy and inflammation' symptom, were selected their depressant effect on mast cells' degranulation and the release amount of histamine in vitro were detected. Among the efficient herbs, Glabrous Greenbrier Rhizome and Paeonia Veitchii showed more rejection capability on mast cells' degranulation as the IC50 of these two kinds of herb were 62.5 μg/mL and 113.8 μg/mL, respectively. Gla-brous Greenbrier Rhizome is worth to be studied due to its conspicuous efficient (the inhibition ratio is 95.63%with the concentration of 500μg/mL) which has not been reported. The mechanism research suggested that protein kinase A may be one of the target point of Glabrous Greenbrier Rhizome's depressant effect on mast cells' de-granulation.