生物灾害科学
生物災害科學
생물재해과학
Jiangxi Plant Protection
2012年
4期
347-351
,共5页
Cry3Aa%蛋白转导结构域(PTD)%甘薯小象甲
Cry3Aa%蛋白轉導結構域(PTD)%甘藷小象甲
Cry3Aa%단백전도결구역(PTD)%감서소상갑
Cry3Aa%TAT protein transduction domain%Cylas formicarius
为探讨 TAT PTD对苏云金杆菌鞘翅目特效基因CryAa是否具有协同作用,本试验在实现了融合基因 TAT PTD-Cry3Aa大肠杆菌原核表达的基础上,以甘薯小象甲为生测对象测定了原核表达产物PTD-Cry3Aa与Cry3Aa的毒力.结果表明,用大肠杆菌表达的Cry3A和PTD-Cry3Aa包涵体初提物均对甘薯小象甲成虫和幼虫有杀虫活性,且表现出一定毒力增效作用,对成虫的增效倍数为2.01倍,对幼虫的增效作用更加明显,达到3.19倍.这一研究结果对于甘薯小象甲的生物防治研究以及转基因抗虫甘薯的研究具有重要的指导作用.
為探討 TAT PTD對囌雲金桿菌鞘翅目特效基因CryAa是否具有協同作用,本試驗在實現瞭融閤基因 TAT PTD-Cry3Aa大腸桿菌原覈錶達的基礎上,以甘藷小象甲為生測對象測定瞭原覈錶達產物PTD-Cry3Aa與Cry3Aa的毒力.結果錶明,用大腸桿菌錶達的Cry3A和PTD-Cry3Aa包涵體初提物均對甘藷小象甲成蟲和幼蟲有殺蟲活性,且錶現齣一定毒力增效作用,對成蟲的增效倍數為2.01倍,對幼蟲的增效作用更加明顯,達到3.19倍.這一研究結果對于甘藷小象甲的生物防治研究以及轉基因抗蟲甘藷的研究具有重要的指導作用.
위탐토 TAT PTD대소운금간균초시목특효기인CryAa시부구유협동작용,본시험재실현료융합기인 TAT PTD-Cry3Aa대장간균원핵표체적기출상,이감서소상갑위생측대상측정료원핵표체산물PTD-Cry3Aa여Cry3Aa적독력.결과표명,용대장간균표체적Cry3A화PTD-Cry3Aa포함체초제물균대감서소상갑성충화유충유살충활성,차표현출일정독력증효작용,대성충적증효배수위2.01배,대유충적증효작용경가명현,체도3.19배.저일연구결과대우감서소상갑적생물방치연구이급전기인항충감서적연구구유중요적지도작용.
In order to study the synergism of the TAT PTD (the protein transduction domain in HIV-1 transactivator of transcription protein) to the Cry3Aa from Bacillus thuringiensis, the fusion gene PTD-Cry3Aa and gene Cry3Aa were expressed in E. coli. cells. The toxicity of prokaryotic expression products from PTD-Cry3Aa and Cry3Aa was tested in sweet potato weevil, Cylas formicarius. The bioassay results showed that the primary extracts of PTD-Cry3Aa and Cry3Aa inclusion bodies exhibited activities against the adults and larvae of sweet potato weevil. The results also indicated there was a synergism of PTD to Cry3Aa both on the adults and the larvae. The synergistic rates were 2.01 folds for the adults while 3.19 folds for the larvae. The study results would play an important role in biological control and transgenic sweet potato against sweet potato weevil.
