CAJ | 학술논문

目的探讨外周血中IL-1β,IL-10,IL-6,TNFα水平与Ki67抗原在不同级别宫颈上皮内瘤变组织中的表达及意义.方法选择于门诊行宫颈刮片细胞学检查及宫颈组织活检的患者,根据细胞学检查结果及病理检查结果,分为3个组,其中正常宫颈的对照组15例,CINⅠ期组15例,CIN Ⅲ期(原位癌)15例.采用酶联免疫吸附测定法(ELISA)检测血清中IL-1β,IL-10,IL-6,TNFα的浓度水平.采用免疫组织化学方法检测宫颈组织中Ki67的表达.结果①对照组中IL-1β,IL-6,TNFα,IL-10水平最低,CINⅢ期(原位癌)组水平最高,三者之间比较差异均有显著性(P<0.05).②在CINⅠ期、CIN Ⅲ期和对照组中,血清IL-1β,IL-10,IL-6,TNFα与Ki67水平之间存在正相关关系(r=0.615,P<0.05;r=0.643,P<0.05;r=0.672,P<0.05);③Ki67抗原表达阳性率在对照组、CINⅠ期及CIN Ⅲ期患者差异有统计学意义(P<0.01).结论①血清IL-1β,IL-6,TNFα,IL-10在诊断宫颈上皮内瘤变的发生方面具有一定价值;②联合运用检测血清IL-1β,IL-10,IL-6,TNFα水平与Ki67抗原阳性表达率对宫颈上皮内瘤变诊断有较好的特异性.
목적탐토외주혈중IL-1β,IL-10,IL-6,TNFα수평여Ki67항원재불동급별궁경상피내류변조직중적표체급의의.방법선택우문진행궁경괄편세포학검사급궁경조직활검적환자,근거세포학검사결과급병리검사결과,분위3개조,기중정상궁경적대조조15례,CINⅠ기조15례,CIN Ⅲ기(원위암)15례.채용매련면역흡부측정법(ELISA)검측혈청중IL-1β,IL-10,IL-6,TNFα적농도수평.채용면역조직화학방법검측궁경조직중Ki67적표체.결과①대조조중IL-1β,IL-6,TNFα,IL-10수평최저,CINⅢ기(원위암)조수평최고,삼자지간비교차이균유현저성(P<0.05).②재CINⅠ기、CIN Ⅲ기화대조조중,혈청IL-1β,IL-10,IL-6,TNFα여Ki67수평지간존재정상관관계(r=0.615,P<0.05;r=0.643,P<0.05;r=0.672,P<0.05);③Ki67항원표체양성솔재대조조、CINⅠ기급CIN Ⅲ기환자차이유통계학의의(P<0.01).결론①혈청IL-1β,IL-6,TNFα,IL-10재진단궁경상피내류변적발생방면구유일정개치;②연합운용검측혈청IL-1β,IL-10,IL-6,TNFα수평여Ki67항원양성표체솔대궁경상피내류변진단유교호적특이성.
Objective To probe into the manifestation and meaning of the levels of IL-1β,IL-10,IL-6,TNFαin peripheral blood and Ki67 antigen in tissues of CIN of different grades .Methods The patients who have gone through the outpatient clinic Pap smear cytologi -cal examination and cervical tissue biopsy were chosen and divided into three groups according to the result of the cytological examination and the pathology examination,15 cases of control group of the normal cervix uteri ,15 cases of CIN period Ⅰ,15 cases of CIN Period Ⅲ(Bow-en).The density level of IL-1β,IL-10,IL-6,TNFαin serum was tested by ELISA and the manifestation of Ki 67 in cervical tissue was exam-ined by the immunohistochemistry method .Results ①In the control group,the level of IL-1β,IL-6,TNFαand IL-10 was the lowest,and that of the IL-1β,IL-6,TNFα,IL-10 in the CINⅢ(carcinoma in situ) was the highest.There was an obvious difference (P<0.05) through the comparison of these three groups.②In the CIN period I,CIN period Ⅲ(carcinoma in situ) and the normal cervical control group ,the IL-1β, IL-10,IL-6,TNFαof serum and the Ki67 level had positive correlationship(r=0.615,P<0.05;r=0.643,P<0.05;r=0.672,P<0.05).③The difference in the percentage of the positive manifestation of Ki 67 antigen in the control group,CIN I and CINⅢhad statistical signifi-cance(P<0.01).Conclusion ①Serum IL-1β,IL-6,TNFα,IL-10 has a certain value in the diagnosis of the occurrence of cervical intraepi -thelial neoplasm.②The combined use of examining the level of serum IL-1β,IL-10,IL-6,TNFαand the percentage of the positive manifesta-tion of Ki67 antigen has a good specificity in the diagnosis of cervical intraepithelial neoplasm .