CAJ | 학술논문

目的探讨肿瘤坏死因子-(TNF-)基因启动子区-308G/A多态性与潍坊地区汉族人群儿童支气管哮喘发病的相关性.方法应用PCR-RFLP方法对91例支气管哮喘患儿(哮喘组)和89例健康儿童(对照组)TNF-基因-308G/A多态性进行检测.结果 TNF-基因-308G/A多态性,GG,GA,AA基因型频率哮喘组分别为83.5%,15.4%,1.1%,对照组分别为92.1%,7.9%,0(χ2=3.54,P>0.05);G和A等位基因频率哮喘组为91.2%和8.8%,对照组为96.1%和3.9%(χ2=3.55,P>0.05).与GG基因型个体比较,GA基因型携带者儿童发生哮喘的风险增加2.16倍(95%CI:1.94~5.55),与G等位基因个体比较,A等位基因携带者儿童发生哮喘的风险显著增加2.35倍(95%CI:2.31~5.71).结论 TNF-基因-308G/A多态性与潍坊地区汉族人群儿童支气管哮喘病的发生无明显相关性.
목적탐토종류배사인자-(TNF-)기인계동자구-308G/A다태성여유방지구한족인군인동지기관효천발병적상관성.방법응용PCR-RFLP방법대91례지기관효천환인(효천조)화89례건강인동(대조조)TNF-기인-308G/A다태성진행검측.결과 TNF-기인-308G/A다태성,GG,GA,AA기인형빈솔효천조분별위83.5%,15.4%,1.1%,대조조분별위92.1%,7.9%,0(χ2=3.54,P>0.05);G화A등위기인빈솔효천조위91.2%화8.8%,대조조위96.1%화3.9%(χ2=3.55,P>0.05).여GG기인형개체비교,GA기인형휴대자인동발생효천적풍험증가2.16배(95%CI:1.94~5.55),여G등위기인개체비교,A등위기인휴대자인동발생효천적풍험현저증가2.35배(95%CI:2.31~5.71).결론 TNF-기인-308G/A다태성여유방지구한족인군인동지기관효천병적발생무명현상관성.
Objective To investigate the association of tumor necrosis factor-(TNF-) gene promoter region-308 G/A polymor-phism with bronchial asthma of children in Weifang Han population .Methods The TNF--308G/A polymorphism was determined by PCR-RFLP in 91 patients with asthma(asthma group) and 89 normal children(control group).Results The frequencies of GG,GA and AA geno-types of TNF--308G/A polymorphism were,respectively,83.5%,15.4%and 1.1%in asthma group,92.1%,7.9%and 0 in control group (χ2 =3.54,P>0.05).The G and A allele frequencies of TNF--308G/A polymorphism were,respectively,91.2% and 8.8% in asthma group,96.1%and 3.9%in control group(χ2 =3.55,P>0.05).No significant differences were found in allele and genotype frequencies of TNF--308G/A between the two groups.The individuals with GA genotype and A allele were 2.16(95%CI:1.94~5.55) (P>0.05) and 2.35(95%CI:2.31~5.71) (P>0.05) times susceptible to asthma than the normal controls ,compared with GG genotype and G allele . Conclusion These results indicated that TNF--308G/A polymorphism did not show association with bronchial asthma of children in Wei -fang population.