CAJ | 학술논문

目的观察盐酸戊乙奎醚对胃蛋白酶误吸所致急性肺损伤大鼠模型肺炎性因子的变化.方法采用气管内注入胃蛋白酶法制作大鼠肺误吸模型.将健康雄性Wistar大鼠45只随机分为3组:空白对照组(C组);生理盐水对照组(NSC 组);盐酸戊乙奎醚组(PHI 组).观察胃蛋白酶注入气管后大鼠平均动脉压(MAP)和心率(HR),用ELISA法测肺泡灌洗液上清和血清中的TNF-α和IL-6的量.光镜观察肺组织病理变化.结果胃蛋白酶法大鼠肺误吸模型建立成功. NSC组HR,MAP先升高后降低;C组HR,MAP较平稳,与NSC组比较,差异均有统计学意义(P<0.01);PHI组经治疗后两者均趋于平稳,与NSC比较差异有统计学意义(P<0.01).除C组外各组大鼠肺泡灌洗液上清和血清中的TNF-α和IL-6两者含量均升高(P<0.01);与NSC组比较,PHI组TNF-α和IL-6含量降低(P<0.05).病理学结果显示:肺组织损伤程度PHI组较NSC组明显减轻.结论盐酸戊乙奎醚可以减轻肺误吸所致急性肺损伤的炎症反应,对胃蛋白酶误吸大鼠肺有保护作用.
목적관찰염산무을규미대위단백매오흡소치급성폐손상대서모형폐염성인자적변화.방법채용기관내주입위단백매법제작대서폐오흡모형.장건강웅성Wistar대서45지수궤분위3조:공백대조조(C조);생리염수대조조(NSC 조);염산무을규미조(PHI 조).관찰위단백매주입기관후대서평균동맥압(MAP)화심솔(HR),용ELISA법측폐포관세액상청화혈청중적TNF-α화IL-6적량.광경관찰폐조직병리변화.결과위단백매법대서폐오흡모형건립성공. NSC조HR,MAP선승고후강저;C조HR,MAP교평은,여NSC조비교,차이균유통계학의의(P<0.01);PHI조경치료후량자균추우평은,여NSC비교차이유통계학의의(P<0.01).제C조외각조대서폐포관세액상청화혈청중적TNF-α화IL-6량자함량균승고(P<0.01);여NSC조비교,PHI조TNF-α화IL-6함량강저(P<0.05).병이학결과현시:폐조직손상정도PHI조교NSC조명현감경.결론염산무을규미가이감경폐오흡소치급성폐손상적염증반응,대위단백매오흡대서폐유보호작용.
Objective To investigate the influence of penehyclidine hydrochloride on pneumonia factor in rats being acute lung injury(ALI)due to Pepsin.Methods Animal models were made in method of instilling pepsin in rats intratracheally .Forty-five healthy male wistar rats were randomly divided into 3 groups:control group(group C);normal saline group(group NSC);penehyclidine hydrochloride group (Group PHI).Mean arterial pressure(MAP) and heart rate(HR)were observed after pepsin injected into the trachea .The amount of TNF-αin and IL-6 in serum and supernatant of lavaging fluid from bronchoalveolar was measured with ELISA .The pulmonary tissue pathological change was investigated by light microscopy .Results Rats'pulmonary pepsin-aspirated model was established successfully .HR and MAP in group NSC elevated then reduced,and group C kept stable relatively,so the differences between them were significant statistically (P<0. 01);HR and MAP both stabilized after treatment in group PHI ,so differences between group PHI and NSC was significant statistically (P<0. 01).Amounts of TNF-αin supernatant and IL-6 in serum increased in all groups except for group C (P<0.01).TNF-αand IL-6 decreased in group PHI compared with group NSC(P<0.05).Pathological results showed:pulmonary tissue injury relieved better in group PHI than NSC obviously.Conclusion These results suggest that penehyclidine hydrochloride can reduce the inflammatory response to ALI and prevent from respiratory failure induced by pepsin aspiration .