CAJ | 학술논문

目的观察锌指结合蛋白(Snail)和上皮性钙粘附蛋白E-cadherin在胃癌组织中的表达,并探讨其意义.方法采用免疫组化SP法检测45例胃癌患者肿瘤组织和20例癌旁正常胃组织中Snail,E-cadherin蛋白的表达.结果胃癌组织中Snail阳性表达率明显高于癌旁正常胃组织(P<0.05),胃癌组织中E-cadherin阳性表达率显著低于癌旁正常胃组织(P<0.05);Snail和E-cad表达均与胃癌的分化程度、浸润深度、临床分期、淋巴结转移、是否有远处器官转移密切相关(P<0.05);Snail与E-cadherin在胃癌组织中的表达呈显著负相关(P<0.05).结论 Snail,E-cadherin的异常表达可能是胃黏膜发生恶性转变及胃癌发生侵袭转移的重要生物学标志,联合检测两种蛋白对预测胃癌的恶性程度及其预后有重要意义.
목적관찰자지결합단백(Snail)화상피성개점부단백E-cadherin재위암조직중적표체,병탐토기의의.방법채용면역조화SP법검측45례위암환자종류조직화20례암방정상위조직중Snail,E-cadherin단백적표체.결과위암조직중Snail양성표체솔명현고우암방정상위조직(P<0.05),위암조직중E-cadherin양성표체솔현저저우암방정상위조직(P<0.05);Snail화E-cad표체균여위암적분화정도、침윤심도、림상분기、림파결전이、시부유원처기관전이밀절상관(P<0.05);Snail여E-cadherin재위암조직중적표체정현저부상관(P<0.05).결론 Snail,E-cadherin적이상표체가능시위점막발생악성전변급위암발생침습전이적중요생물학표지,연합검측량충단백대예측위암적악성정도급기예후유중요의의.
Objective To investigate the expression of Snail and E-cadherin protein in gastric cancer and explore their significance.Methods The expression of Snail and E-cadherin protein was detected by immunohistochemi-cal SP method in 45 cases of gastric carcinoma tissue and 20 cases of para-cancerous normal gastric tissue.Results The positive rate of Snail immunostaining in gastric carcinoma was higher than that in adjacent normal gastric tissue (P<0. 05),the positive rate of E-cadherin immunostaining in gastric cancer was lower than that in adjacent normal gastric tissue (P<0.05).The expression of Snail and E-cadherin in gastric carcinoma tissue was closely related with the depth of tumor invasion,differentiatial degree,clinical stage,lymph node metastases and distant metastases (P<0.05).There was negative correlation between the expression of Snail and E-cadherin protein in gastric carcinoma(P<0.05).Conclusion The abnormal expression of Snail and E-cadherin might be important biological markers for malignant transformation , invasion and metastasis of gastric carcinoma .Co-detection of Snail and E-cadherin has significant effect on predicting the degree of malignancy and prognosis of gastric cancer .