CAJ | 학술논문

利用紫外-可见稳态吸收光谱,稳态荧光发射光谱和激光光解瞬态光谱实验方法研究了磷酸基团在环丙沙星(CPX)光敏损伤DNA中的作用.紫外-可见和稳态荧光光谱实验证实了磷酸根离子影响环丙沙星的稳态吸收和发射谱,实验结果表明磷酸根是通过弱相互作用与环丙沙星结合.我们还利用激光闪光光解实验分别研究了鸟苷(Gua),脱氧鸟苷(dG)以及脱氧鸟苷酸(dGMP)对环丙沙星三线态(3CPX*)的影响,通过对比实验证实了在环丙沙星光敏损伤dGMP中,由于磷酸基团的存在,导致了环丙沙星三线态吸收峰的改变,从而改变了光敏损伤反应的途径.通过研究发现,光敏损伤途径的改变是由于dGMP结构上磷酸基团通过氢键与环丙沙星结合所造成的.最后,根据实验结果并对比Gua, dG和dGMP的结构,提出了一个合理的磷酸基团的作用机理.
이용자외-가견은태흡수광보,은태형광발사광보화격광광해순태광보실험방법연구료린산기단재배병사성(CPX)광민손상DNA중적작용.자외-가견화은태형광광보실험증실료린산근리자영향배병사성적은태흡수화발사보,실험결과표명린산근시통과약상호작용여배병사성결합.아문환이용격광섬광광해실험분별연구료조감(Gua),탈양조감(dG)이급탈양조감산(dGMP)대배병사성삼선태(3CPX*)적영향,통과대비실험증실료재배병사성광민손상dGMP중,유우린산기단적존재,도치료배병사성삼선태흡수봉적개변,종이개변료광민손상반응적도경.통과연구발현,광민손상도경적개변시유우dGMP결구상린산기단통과경건여배병사성결합소조성적.최후,근거실험결과병대비Gua, dG화dGMP적결구,제출료일개합리적린산기단적작용궤리.
Stable and transient spectroscopic studies were conducted to investigate the influence of DNA phosphate bases in the process of DNA damage photo-induced by ciprofloxacin (CPX). The results of UV-visible and fluorescence studies confirmed that the absorption and emission properties of CPX were affected by the concentration of phosphate buffer (PB), and these results indicated that there are interactions between CPX and the phosphate anion. To investigate the relationship between the phosphate base and CPX, a comparative experiment was conducted using guanosine (Gua), 2?-deoxyguanosine (dG), and 2?-deoxyguanosine-5?-monophosphate (dGMP). By comparative experiments, we found that the type of spectrum of CPX triplet state was changed due to the phosphate base of dGMP, hence, we concluded that the photo-damage process was changed. The effects of phosphate base of dGMP on the CPX triplet state (3CPX*) spectrum have been investigated by laser flash photolysis methods, and the results confirmed that there are hydrogen-bonding interactions between the phosphate base of dGMP and CPX. In this study, we found that CPX and dGMP are combined together by the hydrogen bond which changed the mode of DNA damage photo-induced by CPX. Final y, based on the results obtained in this study, a rational scheme to describe the role of phosphate base playing in the DNA damage process photo-induced by CPX is proposed.