CAJ | 학술논문

提出了一种基于偏振滤波图像增强和动态散斑照明的新型宽场荧光层析显微镜.该显微镜采用了一种新型的偏振滤波图像增强技术,基于激发光与荧光偏振态的差异,利用偏振器件滤除激发光;并利用动态散斑照明实现宽场层析.该荧光层析显微镜具有结构简单、低成本、响应速度快、容易操作等特点.实验研究结果表明,本文提出的滤波方案能够显著地提高图像质量,利用动态散斑照明实现宽场层析具有较高的纵向分辨能力.研究丰富了在荧光显微镜中,从强激发光中提取弱荧光信号的技术手段,为今后发展具有快速响应,波长可调谐的多光谱荧光层析等高端的显微镜具有重要参考意义.
제출료일충기우편진려파도상증강화동태산반조명적신형관장형광층석현미경.해현미경채용료일충신형적편진려파도상증강기술,기우격발광여형광편진태적차이,이용편진기건려제격발광;병이용동태산반조명실현관장층석.해형광층석현미경구유결구간단、저성본、향응속도쾌、용역조작등특점.실험연구결과표명,본문제출적려파방안능구현저지제고도상질량,이용동태산반조명실현관장층석구유교고적종향분변능력.연구봉부료재형광현미경중,종강격발광중제취약형광신호적기술수단,위금후발전구유쾌속향응,파장가조해적다광보형광층석등고단적현미경구유중요삼고의의.
We propose a novel wide-field fluorescence sectioning microscope based on polarization filtering image enhancement and dynamic speckle illumination in this article. A new type of polarization filtering image enhancement technology, based on the differences in polarization characteristic between excitation light and fluorescence, is used to filter out the excitation light and enhance image quality. The dynamic speckle illumination is employed to achieve the wide field fluorescence sectioning which has many advantages, such as simple configuration, low cost, rapid response and easy operation. The experimental results indicate that our filtering method can be utilized to improve the image quality significantly, and the dynamic using speckle illumination can be employed to achieve optical sectioning with a wide field and a high vertical resolution capability. Our study is not only to enrich the technological method to extract the weak fluorescence signal from the strong excitation light, but also to provide a significant reference for developing a light wavelength tunable multi-spectral fluorescence microscope with rapid response.