物理学报
物理學報
물이학보
2013年
3期
168-172
,共5页
王淑莹%章海军%张冬仙*
王淑瑩%章海軍%張鼕仙*
왕숙형%장해군%장동선*
微球%显微成像%毛细管–微球组合探针%高分辨
微毬%顯微成像%毛細管–微毬組閤探針%高分辨
미구%현미성상%모세관–미구조합탐침%고분변
microsphere%microscopic imaging%micropipette-microsphere combined probe%high-resolution
提出和发展了基于毛细管–微球组合探针的任选区、高分辨显微成像新方法.建立了微球显微成像的物理模型,利用成像理论,推导出微球成像的放大倍率;采用3.0,4.4,5.6,7.5,10.0μm等不同直径的SiO2微球,对未经刻录的DVD光盘进行了微球显微成像实验,可以观察到DVD光盘的微纳米结构被明显放大且对比度显著提高,与理论计算结果相符合;采用毛细管微探针操纵微球的方法,实现了基于微球透镜阵列的样品微纳米结构的高分辨显微成像;在此基础上,进一步将毛细管微探针与微球组合,制备出毛细管–微球组合型探针,首次实现了基于微球透镜的样品任意区域高分辨显微成像.
提齣和髮展瞭基于毛細管–微毬組閤探針的任選區、高分辨顯微成像新方法.建立瞭微毬顯微成像的物理模型,利用成像理論,推導齣微毬成像的放大倍率;採用3.0,4.4,5.6,7.5,10.0μm等不同直徑的SiO2微毬,對未經刻錄的DVD光盤進行瞭微毬顯微成像實驗,可以觀察到DVD光盤的微納米結構被明顯放大且對比度顯著提高,與理論計算結果相符閤;採用毛細管微探針操縱微毬的方法,實現瞭基于微毬透鏡陣列的樣品微納米結構的高分辨顯微成像;在此基礎上,進一步將毛細管微探針與微毬組閤,製備齣毛細管–微毬組閤型探針,首次實現瞭基于微毬透鏡的樣品任意區域高分辨顯微成像.
제출화발전료기우모세관–미구조합탐침적임선구、고분변현미성상신방법.건립료미구현미성상적물리모형,이용성상이론,추도출미구성상적방대배솔;채용3.0,4.4,5.6,7.5,10.0μm등불동직경적SiO2미구,대미경각록적DVD광반진행료미구현미성상실험,가이관찰도DVD광반적미납미결구피명현방대차대비도현저제고,여이론계산결과상부합;채용모세관미탐침조종미구적방법,실현료기우미구투경진렬적양품미납미결구적고분변현미성상;재차기출상,진일보장모세관미탐침여미구조합,제비출모세관–미구조합형탐침,수차실현료기우미구투경적양품임의구역고분변현미성상.
In this paper, we propose a novel location-free high-resolution optical microscopic imaging method based on micropipette-microsphere combined probe. According to optical principle, a physical model is established to determine the value of magnification. With SiO2 microspheres of different diameters such as 3.0, 4.4, 5.6, 7.5 and 10.0 μm, microscopic imaging experiments are carried out. The results show that the microstructure of un-etched DVD disk can be significantly magnified and the image contrast is ob-viously improved, which is consistent well with the theoretical calculation. Microscopic imaging of microstructures based on SiO2 microsphere array is realized by controlling the microspheres with a micropipette. Furthermore, a kind of micropipette-microsphere combined probe is prepared, by which location-free high-resolution optical microscopic imaging has implemented for the first time.
