CAJ | 학술논문

光热效应是激光与生物组织相互作用中的一个主要因素,但其产生、传输和作用机理尚不十分清晰.本文采用双波长近红外激光辐照和膜片钳技术相结合的方法,选择980 nm和845 nm两个波长的近红外激光,因其在水中的吸收系数分别为0.502 cm?1和0.0378 cm?1,接近十倍差异.若溶液是产生光热响应的主要介导物质,则期望这两个波长的激光辐照所产生的溶液温升也将呈现相应的十倍比例关系.研究中把溶液光热响应过程分为温升的建立和耗散两个阶段.在温升建立阶段,理论模型的建立采用长时程(激光作用时程长于介质的热弛豫时间)作用理论的研究结果,实验是使用膜片钳系统来测量细胞外溶液中,已进行温度标定的、充灌溶液的玻璃微电极电导变化,根据这个电导变化来定量研究溶液光热响应与其吸收特性的关联性;在耗散阶段,使用膜片钳系统监测神经细胞的电生理功能变化.理论和实验两方面的结果都表明,溶液对低强度近红外激光的吸收特性决定了其光热响应.这一结果,可以直接用于生物组织光热响应特性相关的机理研究.
광열효응시격광여생물조직상호작용중적일개주요인소,단기산생、전수화작용궤리상불십분청석.본문채용쌍파장근홍외격광복조화막편겸기술상결합적방법,선택980 nm화845 nm량개파장적근홍외격광,인기재수중적흡수계수분별위0.502 cm?1화0.0378 cm?1,접근십배차이.약용액시산생광열향응적주요개도물질,칙기망저량개파장적격광복조소산생적용액온승야장정현상응적십배비례관계.연구중파용액광열향응과정분위온승적건립화모산량개계단.재온승건립계단,이론모형적건립채용장시정(격광작용시정장우개질적열이예시간)작용이론적연구결과,실험시사용막편겸계통래측량세포외용액중,이진행온도표정적、충관용액적파리미전겁전도변화,근거저개전도변화래정량연구용액광열향응여기흡수특성적관련성;재모산계단,사용막편겸계통감측신경세포적전생리공능변화.이론화실험량방면적결과도표명,용액대저강도근홍외격광적흡수특성결정료기광열향응.저일결과,가이직접용우생물조직광열향응특성상관적궤리연구.
Photothermal effect has been proved to mediate the interaction of near-infrared laser with biological tissue. However, the gen-eration and transformation mechanism of the photothermal effect is still unclear. In this paper, we combine a patch clamp technique with the laser simulation to figure out the chromophores, which are responsible for the photothermal effect generation. This method is based on the fact that temperature dependence of solution can be measured as resistance changes. A dual-wavelength infrared light irradiating the open pipette in extracellular solution is designed to study the relation between the photothermal effect and the absorption property of solution. The principle is based on that the nearly ten times difference in the magnitude of the optical absorption coefficient in water (0.502 cm?1 at 980 nm and 0.0378 cm?1 at 845 nm), makes the corresponding proportional absorption-driven temperature rise. The photothermal effect in laser-tissue interaction can be assessed in two stages: the establishment and the dissipation of the temperature rise. In the establishment stage, an open pipette method is employed to measure the temperature rise by fabricating a glass pipette which is filled with electrolyte solution. In the dissipation stage, the electrophysiological function of a living neuron cell is studied based on a patch clamp. Theoretical calculation and experimental results show that the optical absorption properties of solution determine the photothermal effect. The results can be used to study the photothermal effect in laser-tissue interaction.