物理学报
物理學報
물이학보
2013年
7期
351-355
,共5页
周恒为%刘君%雷婷%黄以能
週恆為%劉君%雷婷%黃以能
주항위%류군%뢰정%황이능
力学谱%蛋白质水凝胶%蛋白质变性
力學譜%蛋白質水凝膠%蛋白質變性
역학보%단백질수응효%단백질변성
mechanical spectrum%protein hydrogel%protein denaturation
本文用液态簧振动力学谱(RMS-L)方法,对典型蛋白质水凝胶鸡蛋清的脱水变性过程进行了测量,结果表明,随水含量的减少,鸡蛋清至少存在4个力学谱的显著变化过程.基于此结果并结合力学谱的理论分析,作者推测,随水含量的减少,鸡蛋清可能依次存在下述4个状态:1)类体水(bulk-likewater)的蛋白质水凝胶态;2)键合水(bond water)的蛋白质水凝胶态;3)键合水和键合蛋白质(bonding protein)的混合态;4)键合蛋白质态.而蛋白质的空间构型(spatial configuration)转变即变性,主要发生在拥有键合水的蛋白质通过失水向键合蛋白质转变的混合态.这表明RMS-L对鸡蛋清脱水变性过程的检测是有效的,所得结果对蛋白质变性机理、以及蛋白质水凝胶态的深入研究也应具有参考价值.
本文用液態簧振動力學譜(RMS-L)方法,對典型蛋白質水凝膠鷄蛋清的脫水變性過程進行瞭測量,結果錶明,隨水含量的減少,鷄蛋清至少存在4箇力學譜的顯著變化過程.基于此結果併結閤力學譜的理論分析,作者推測,隨水含量的減少,鷄蛋清可能依次存在下述4箇狀態:1)類體水(bulk-likewater)的蛋白質水凝膠態;2)鍵閤水(bond water)的蛋白質水凝膠態;3)鍵閤水和鍵閤蛋白質(bonding protein)的混閤態;4)鍵閤蛋白質態.而蛋白質的空間構型(spatial configuration)轉變即變性,主要髮生在擁有鍵閤水的蛋白質通過失水嚮鍵閤蛋白質轉變的混閤態.這錶明RMS-L對鷄蛋清脫水變性過程的檢測是有效的,所得結果對蛋白質變性機理、以及蛋白質水凝膠態的深入研究也應具有參攷價值.
본문용액태황진동역학보(RMS-L)방법,대전형단백질수응효계단청적탈수변성과정진행료측량,결과표명,수수함량적감소,계단청지소존재4개역학보적현저변화과정.기우차결과병결합역학보적이론분석,작자추측,수수함량적감소,계단청가능의차존재하술4개상태:1)류체수(bulk-likewater)적단백질수응효태;2)건합수(bond water)적단백질수응효태;3)건합수화건합단백질(bonding protein)적혼합태;4)건합단백질태.이단백질적공간구형(spatial configuration)전변즉변성,주요발생재옹유건합수적단백질통과실수향건합단백질전변적혼합태.저표명RMS-L대계단청탈수변성과정적검측시유효적,소득결과대단백질변성궤리、이급단백질수응효태적심입연구야응구유삼고개치.
Protein denaturation is not only one of the basic problems in biophysics and biochemistry, but also a practical one in applications. It is undoubtedly useful to explore new methods for detecting protein denaturation because they will surely provide some new information from new angles of view. For the first time, Sofar as we know we apply the reed-vibration mechanical spectroscopy for liquids (RMS-L) to measure the dehydration and denaturation process of egg white, one of typical protein hydrogels, in this work. The results show that there exist at least 4 remarkable processes of mechanical spectra with the reduction of water content. Based on the experiments and the analyses according to the relevant mechanical spectrum theories, the authors inferred that, with the water content reduction egg white may undergo the following 4 states successively: 1) bulk-like water protein hydrogel state;2) bond water protein hydrogel state;3) bond water and bonding protein mixed state;4) bonding protein state. And the spatial configuration change of protein, namely degeneration, happens mainly in the mixed state in which the protein with bond water transforms to binding protein by losing the water. This means that the detection of dehydration and denaturation of egg white by RMS-L is effective, and we think the conclusions would also be reference materials for the deep studies of protein denaturation mechanisms and protein hydrogel states.