CAJ | 학술논문

背景:骨髓间充质干细胞具有广泛的应用前途,其三系分化的能力和免疫诱导特性使其具有再生医学的应用前景.目的:从小鼠骨髓中研究分离制备Flk-1+间充质干细胞的新方法,检测了这类细胞的生物特性.方法:以小鼠骨髓为研究对象,分离单个核细胞,体外培养并扩增原始间充质干细胞,检测它们的细胞周期、表型和多系分化的能力.结果与结论:小鼠来源的骨髓来源的原始间充质干细胞呈成纤维样生长,大部分细胞处于G /G01期,并且高表达Flk1, CD13,CD29,CD44,小鼠骨髓来源Flk-1+间充质干细胞在光镜下均呈成纤维样或多角型贴壁生长,在体外相应的诱导液环境中均可以向三系分化.提示小鼠骨髓来源Flk-1+间充质干细胞具有多向分化潜能.
배경:골수간충질간세포구유엄범적응용전도,기삼계분화적능력화면역유도특성사기구유재생의학적응용전경.목적:종소서골수중연구분리제비Flk-1+간충질간세포적신방법,검측료저류세포적생물특성.방법:이소서골수위연구대상,분리단개핵세포,체외배양병확증원시간충질간세포,검측타문적세포주기、표형화다계분화적능력.결과여결론:소서래원적골수래원적원시간충질간세포정성섬유양생장,대부분세포처우G /G01기,병차고표체Flk1, CD13,CD29,CD44,소서골수래원Flk-1+간충질간세포재광경하균정성섬유양혹다각형첩벽생장,재체외상응적유도액배경중균가이향삼계분화.제시소서골수래원Flk-1+간충질간세포구유다향분화잠능.
BACKGROUND:The bone marrow mesenchymal stem cel s can be widely used, the three-lineage differentiation capacity and immune induction characteristics make it widely used in regenerative medicine. OBJECTIVE:To investigate the new method to isolate the Flk-1+mesenchymal stem cel s from mouse bone marrow, and to detect the biological characteristics of Flk-1+mesenchymal stem cel s. METHODS:The mononuclear cel s were isolated from the bone marrow and the original mesenchymal stem cel s were in vitro cultured and amplified. The cel cycle, phenotype and multilineage differentiation capacity were detected. RESULTS AND CONCLUSION:The original mesenchymal stem cel s derived from mice bone marrow were fibrolbast like, and most of them stayed in G0/G1 phase. Cel phenotype test showed they could highly express Flk1, CD13, CD29, CD44;the bone marrow-derived Flk-1+mesenchymal stem cel s under light microscope showed a fibroblast-like or polygonal adherent growth, and under certain circumstances, they exhibited osteogenic, adipogenic and chondrogenic differentiation. Bone marrow-derived Flk-1+mesenchymal stem cel s have multilineage differentiation potential.