CAJ | 학술논문

背景:器官移植耐受的最佳效果是能够诱导对移植抗原的特异性免疫耐受.目的:探讨小鼠异基因皮肤移植后,通过受体同基因造血干细胞移植重建免疫系统诱导移植皮肤免疫耐受的可行性.方法:取BALB/c小鼠骨髓.以C57BL/6小鼠为供体,BALB/c小鼠为受体,进行异基因皮肤移植;32只受体鼠随机均分为4组:移植对照组、环孢素A组、照射组和骨髓移植组.结果与结论:照射组小鼠10 d内全部死亡,外周血白细胞数呈持续性降低;而骨髓移植组小鼠长期存活,白细胞数全身照射后6 d降到最低,之后持续性增高,照射后21 d与环孢素A组比较差异无显著性意义(P >0.05),移植皮肤存活时间显著长于其他各组(P<0.01),其淋巴细胞浸润及组织结构破坏明显减少,小鼠脾细胞对供体小鼠脾细胞增殖反应显著降低.说明同基因骨髓细胞移植重建免疫系统可显著延长小鼠移植皮肤存活时间,可诱导供者特异性免疫耐受.
배경:기관이식내수적최가효과시능구유도대이식항원적특이성면역내수.목적:탐토소서이기인피부이식후,통과수체동기인조혈간세포이식중건면역계통유도이식피부면역내수적가행성.방법:취BALB/c소서골수.이C57BL/6소서위공체,BALB/c소서위수체,진행이기인피부이식;32지수체서수궤균분위4조:이식대조조、배포소A조、조사조화골수이식조.결과여결론:조사조소서10 d내전부사망,외주혈백세포수정지속성강저;이골수이식조소서장기존활,백세포수전신조사후6 d강도최저,지후지속성증고,조사후21 d여배포소A조비교차이무현저성의의(P >0.05),이식피부존활시간현저장우기타각조(P<0.01),기림파세포침윤급조직결구파배명현감소,소서비세포대공체소서비세포증식반응현저강저.설명동기인골수세포이식중건면역계통가현저연장소서이식피부존활시간,가유도공자특이성면역내수.
BACKGROUND:The optimal effect of organ transplantation tolerance is to induce the specific immunologic tolerance to transplantation antigens. OBJECTIVE:To study the possibility of using syngeneic hematopoietic stem cel s to reconstitute immune system and induce transplantation tolerance to ful y mismatched skin al ografts in mice. METHODS:BALB/c mouse marrow was taken. Syngeneic skin transplantation was performed taking C57BL/6 mice as donors and BALB/c mice as recipients. Thirty-two BALB/c mice were randomly divided into four groups:control, cyclosporin A, cyclosporin A+total body irradiation, cyclosporin A+total body irradiation, cyclosporin A+total body irradiation+bone marrow transplantation. RESULTS AND CONCLUSION:In the cyclosporin A+total body irradiation group, al mice died within 10 days and the counts of peripheral blood white blood cel s were persistently declined. In the cyclosporin A+total body irradiation+bone marrow transplantation group, mice survived during the experimentation, the counts of peripheral blood white blood cel s decreased to the bottom after 6 days of irradiation and then rebounded, and increased to the level of the cyclosporine A group after 21 days of irradiation (P>0.05). The average survival time of skin grafts in the cyclosporin A+total body irradiation+bone marrow transplantation group was significantly longer than that in the other groups (P<0.01). There were less infiltrated cel s and less destructed tissue, and decreased proliferation of recipient mouse spleen cel s in the cyclosporin A+total body irradiation+bone marrow transplantation group than in the other groups. These findings suggest that immune reconstitution with syngeneic bone marrow cel s can obviously prolong the survival time of mouse skin al ografts and induce donor-specific tolerance.