CAJ | 학술논문

背景:有研究表明脑源性神经营养因子可以维持神经元的存活、影响神经元的迁移,在体外可以促进神经干细胞的存活和分化.目的:探讨脑源性神经营养因子对低血糖幼鼠海马神经干细胞定向分化的作用.方法:取新生1 d低血糖模型大鼠脑海马组织进行原代、传代及单细胞克隆培养.培养的细胞一部分进行神经干细胞鉴定,另一部分依据培养液中脑源性神经营养因子质量浓度的不同将单克隆细胞分为0,100,200μg/L组,取第4代细胞进行诱导分化,行神经元特异性烯醇化酶免疫荧光染色,计数阳性细胞比例.结果与结论:单克隆培养后3组细胞均呈巢蛋白阳性,诱导分化后细胞呈行神经元特异性烯醇化酶和胶质纤维酸性蛋白阳性;100,200μg/L组神经干细胞生长较快,且分化为神经元特异性烯醇化酶阳性细胞比例较高(P<0.05),但两组神经干细胞之间差异无显著性意义(P >0.05).提示脑源性神经营养因子促进低血糖幼鼠海马神经干细胞向神经元定向分化.
배경:유연구표명뇌원성신경영양인자가이유지신경원적존활、영향신경원적천이,재체외가이촉진신경간세포적존활화분화.목적:탐토뇌원성신경영양인자대저혈당유서해마신경간세포정향분화적작용.방법:취신생1 d저혈당모형대서뇌해마조직진행원대、전대급단세포극륭배양.배양적세포일부분진행신경간세포감정,령일부분의거배양액중뇌원성신경영양인자질량농도적불동장단극륭세포분위0,100,200μg/L조,취제4대세포진행유도분화,행신경원특이성희순화매면역형광염색,계수양성세포비례.결과여결론:단극륭배양후3조세포균정소단백양성,유도분화후세포정행신경원특이성희순화매화효질섬유산성단백양성;100,200μg/L조신경간세포생장교쾌,차분화위신경원특이성희순화매양성세포비례교고(P<0.05),단량조신경간세포지간차이무현저성의의(P >0.05).제시뇌원성신경영양인자촉진저혈당유서해마신경간세포향신경원정향분화.
BACKGROUND: There is evidence that brain-derived neurotrophic factor can maintain neuronal survival, influence neuronal migration, and promote the survival and differentiation of neural stem cel s in vitro. OBJECTIVE:To investigate the effects of brain-derived neurotrophic factor on the directional differentiation of hypoglycemia neonatal rat hippocampal neural stem cel s. METHODS:Neonatal 1-day hypoglycemia rat hippocampal tissue was harvested and subjected to primary culture, subculture and cel monoclonal culture. Then cel s were divided into two parts:one part was used for identification of neural stem cel s, and the other part was divided into three groups as per different concentrations of brain-derived neurotrophic factors (0, 100, 200μg/L). Passage 4 cel s were used for induced differentiation. Neuron-specific enolase immunofluorescence staining was used for neuronal identification and positive cel s were counted. RESULTS AND CONCLUSION:After monoclonal culture, three groups of cel s were positive for nestin. After induced differentiation, cel s were positive for neuron-specific enolase and glial fibril ary acidic protein staining. Neural stem cel s in the 100μg/L and 200μg/L groups grew faster, and produced higher proportion of neuron-specific enolase-positive cel s than those in the 0μg/L group (P<0.05). But there was no significant difference between 100μg/L group and 200μg/L group. These findings suggest that brain-derived neurotrophic factor promotes the directional differentiation of hypoglycemia neonatal rat hippocampal neural stem cel s.