CAJ | 학술논문

背景:大量研究证实脐血间充质干细胞体外培养可被诱导分化为表达神经元和神经胶质细胞表面标志物的细胞,对神经系统疾病起到替代修复的作用.目的:观察人脐血单个核细胞移植治疗痉挛型小儿脑性瘫痪的疗效和安全性.方法:收集正常足月剖腹产胎儿的脐血,经肝素抗凝,用淋巴细胞分离液分离脐血单个核细胞,采用差速贴壁法进行纯化,并经腰椎穿刺注入30例痉挛型脑性瘫痪患者体内.结果与结论:移植后患者肢体的肌肉痉挛程度和关节活动度明显改善,与移植前相比差异有显著性意义(P<0.05),移植后和随访过程中无不良反应和并发症.
배경:대량연구증실제혈간충질간세포체외배양가피유도분화위표체신경원화신경효질세포표면표지물적세포,대신경계통질병기도체대수복적작용.목적:관찰인제혈단개핵세포이식치료경련형소인뇌성탄탄적료효화안전성.방법:수집정상족월부복산태인적제혈,경간소항응,용림파세포분리액분리제혈단개핵세포,채용차속첩벽법진행순화,병경요추천자주입30례경련형뇌성탄탄환자체내.결과여결론:이식후환자지체적기육경련정도화관절활동도명현개선,여이식전상비차이유현저성의의(P<0.05),이식후화수방과정중무불량반응화병발증.
BACKGROUND:A large number of studies have demonstrated that umbilical cord mesenchymal stem cel s cultured in vitro can express the surface markers of neurons and glial cel s under induction and exhibit replacing and repairing effects for nervous system disease. OBJECTIVE:To study the therapeutic effect and safety of transplantation of human umbilical cord blood mononuclear cel s in treatment of infantile spastic cerebral palsy. METHODS:Umbilical cord blood was harvested from ful term deliveries scheduled for cesarean section and anti-coagulated with heparin. Umbilical cord blood mononuclear cel s were isolated by lymphocyte separation medium, purified by Nash differential adhesion, and then injected into subarachnoid cavity of 30 infantile patients with spastic cerebral palsy. RESULTS AND CONCLUSION:Compared with prior to treatment, the muscle spastic and joint range of motion significantly improved after treatment (P<0.05). There were no adverse events and complications after transplantation and during fol ow-up period.