CAJ | 학술논문

背景:研究发现环氧化酶2抑制剂具有预防肘关节周围异位骨化的作用.目的:观察环氧化酶2、骨形态形成蛋白2和血管内皮生长因子在肘关节创伤后异位骨化组织中的表达,并分析其相关性.方法:采用免疫组化SP法检测18例肘关节创伤后异位骨化组织及10例正常骨组织中环氧化酶2、骨形态形成蛋白2和血管内皮生长因子的表达水平,利用HPIAS-1000图像分析系统测定异位骨化组织与正常骨组织中环氧化酶2、骨形态形成蛋白2和血管内皮生长因子的平均吸光度和阳性区域面积百分率,并分析3种蛋白阳性区域面积百分率之间的相关性.结果与结论:异位骨化组织中环氧化酶2、骨形态形成蛋白2和血管内皮生长因子呈高表达;正常骨组织中呈低表达或不表达.图像分析结果显示异位骨化组织中3种蛋白平均吸光度及阳性区域面积百分率显著高于正常骨组织(P <0.01).异位骨化组织中环氧化酶2与骨形态形成蛋白2、血管内皮生长因子的阳性区域面积百分率呈正相关(P<0.01).提示环氧化酶2、骨形态形成蛋白2和血管内皮生长因子在异位骨化的形成过程中起重要作用,环氧化酶2可能通过诱导骨形态形成蛋白2和血管内皮生长因子的表达从而促进异位骨化组织中的成骨和血管形成.
배경:연구발현배양화매2억제제구유예방주관절주위이위골화적작용.목적:관찰배양화매2、골형태형성단백2화혈관내피생장인자재주관절창상후이위골화조직중적표체,병분석기상관성.방법:채용면역조화SP법검측18례주관절창상후이위골화조직급10례정상골조직중배양화매2、골형태형성단백2화혈관내피생장인자적표체수평,이용HPIAS-1000도상분석계통측정이위골화조직여정상골조직중배양화매2、골형태형성단백2화혈관내피생장인자적평균흡광도화양성구역면적백분솔,병분석3충단백양성구역면적백분솔지간적상관성.결과여결론:이위골화조직중배양화매2、골형태형성단백2화혈관내피생장인자정고표체;정상골조직중정저표체혹불표체.도상분석결과현시이위골화조직중3충단백평균흡광도급양성구역면적백분솔현저고우정상골조직(P <0.01).이위골화조직중배양화매2여골형태형성단백2、혈관내피생장인자적양성구역면적백분솔정정상관(P<0.01).제시배양화매2、골형태형성단백2화혈관내피생장인자재이위골화적형성과정중기중요작용,배양화매2가능통과유도골형태형성단백2화혈관내피생장인자적표체종이촉진이위골화조직중적성골화혈관형성.
BACKGROUND:Several studies have shown that cyclooxygenase 2 (COX-2) inhibitors can prevent heterotopic ossification around the elbow joint. OBJECTIVE:To investigate the expression of COX-2, bone morphogenetic protein 2 (BMP-2) and vascular endothelial growth factor (VEGF) in post-traumatic elbow heterotopic ossification tissues, and to analyze their relationship. METHODS:The expressions of COX-2, BMP-2 and VEGF were examined by S-P immunohistochemical staining in 18 cases of heterotopic ossification and 10 cases of normal bone tissue. The 10 cases were as controls. The average absorbance value and the percentage of positive area of COX-2, BMP-2 and VEGF in heterotopic ossification and normal bone tissue were measured by HPIAS-1000 image analysis system. The relationship among the percentage of the positive area in the three kinds of proteins was analyzed. RESULTS AND CONCLUSION:COX-2 ,BMP-2 and VEGF were strongly expressed in heterotopic ossification,but those in the normal bone tissue showed low express or no express. Image analysis demonstrated that the average absorbance value and the percentage of positive area of COX-2, BMP-2 and VEGF in heterotopic ossification were significantly higher than those in the normal bone tissue (P<0.01). The percentage of positive area of COX-2 expression was closely correlated with that in the BMP-2 and VEGF in heterotopic ossification (P<0.01). These findings suggest