CAJ | 학술논문

背景:研究表明,低氧会引起骨折延迟愈合或不愈合,骨密度减低,使骨质疏松、骨折等疾病的发病率升高.成骨细胞是骨形成、生长和发育主要的功能细胞.目的:观察缺氧对体外培养成骨细胞增殖、分化及基因表达的影响.方法:选用新生Wistar大鼠颅盖骨,使用胰酶-胶原酶序贯消化法获取成骨细胞,进行体外传代培养及鉴定.在缺氧培养下应用MTT法测定成骨细胞增殖率,对硝基苯磷酸盐法测定成骨细胞碱性磷酸酶活性,反转录-聚合酶链反应法测定成骨细胞内骨钙素及Ⅰ型胶原的表达.结果与结论:缺氧具有抑制成骨细胞增殖,降低碱性磷酸酶活性及下调大鼠成骨细胞中Ⅰ型胶原α1、骨钙素基因表达的作用,随缺氧时间的增加作用更加明显.提示缺氧可通过抑制成骨细胞增殖、分化成熟及下调Ⅰ型胶原α1、骨钙素基因表达而降低成骨能力,从而促进骨质疏松的发生.
배경:연구표명,저양회인기골절연지유합혹불유합,골밀도감저,사골질소송、골절등질병적발병솔승고.성골세포시골형성、생장화발육주요적공능세포.목적:관찰결양대체외배양성골세포증식、분화급기인표체적영향.방법:선용신생Wistar대서로개골,사용이매-효원매서관소화법획취성골세포,진행체외전대배양급감정.재결양배양하응용MTT법측정성골세포증식솔,대초기분린산염법측정성골세포감성린산매활성,반전록-취합매련반응법측정성골세포내골개소급Ⅰ형효원적표체.결과여결론:결양구유억제성골세포증식,강저감성린산매활성급하조대서성골세포중Ⅰ형효원α1、골개소기인표체적작용,수결양시간적증가작용경가명현.제시결양가통과억제성골세포증식、분화성숙급하조Ⅰ형효원α1、골개소기인표체이강저성골능력,종이촉진골질소송적발생.
BACKGROUND:Several researches have shown that hypoxia can lead to the healing of fracture delayed or non healing, and reduce bone density, which wil improve the incidence of osteoporosis and fracture. OBJECTIVE:To investigate the effects of hypoxia on the proliferation, differentiation and gene expression of osteoblasts cultured in vitro. METHODS:The cranium from a newborn Wistar rat was col ected and osteoblasts were extracted by trypsogen-col agenase sequential digestion method. The cel s were subcultured in vitro and identified. The reproductive rate of osteoblasts was tested by MTT assay. Alkaline phosphatase activity of osteoblasts was detected by nitrophenylphosphate method. Bone gamma-carboxyglutamic-acid-containing proteins (BGP) and type col agenⅠexpression were measured by reverse transcription-PCR method. RESULTS AND CONLUSION:Our studies revealed that hypoxia could inhibit the proliferation of osteoblasts and reduce alkaline phosphatase activity as wel as decrease the expression of BGP mRNA and type col agen mRNA in aⅠtime-dependent manner. These findings suggest that hypoxia can inhibit the proliferation and differentiation of osteoblasts cultured in vitro and decrease the expression of BGP mRNA and type col agen, which wil decreaseⅠosteogenic ability and promote the incidence of osteoporosis.