CAJ | 학술논문

背景:小口径组织工程血管的远期结果研究极少,尚未见到研究组织工程血管分子水平、离子水平远期结果和平滑肌细胞与钙化关系的报道.目的:利用脱细胞猪股动脉基质作为支架和犬血管壁细胞作为种子细胞体外构建小口径组织工程血管,植入种子细胞供体犬股动脉部位6个月,观察植入物中层平滑肌细胞与钙化的关系.方法:12只实验犬被随机分为支架组(n=6)和再细胞化组(n=6),自体股动脉被作为对照组;支架组犬接受猪股动脉经脱细胞后的基质支架植入双侧股动脉,再细胞化组犬接受受体血管壁细胞共同培养、联合种植于脱细胞的猪股动脉基质并体外预适应后植入血管壁细胞供体双侧股动脉位置;6个月后测定植入物和对照组股动脉组织钙含量、平滑肌密度和病理学变化.结果与结论:小口径组织工程血管植入后6个月检查见2组植入物无明显狭窄和扩张,扫描电镜示内表面均已完全内皮化,有管壁僵硬和局部钙化斑块形成,以上改变以支架组植入物更明显.支架组管道组织钙含量显著高于再细胞化组和自体股动脉(P <0.01),再细胞化组植入物组织钙含量亦显著高于自体股动脉(P <0.01);病理学检查示再细胞化组植入物平滑肌密度高于支架组(P<0.01),再细胞化组和支架组植入物平滑肌密度均低于对照组(P<0.01);超声检查见2组管道植入术后即刻与6个月后舒缩幅度较邻近自体股动脉舒缩幅度小,有部分管道无舒缩功能.结果提示,猪股动脉常规脱细胞方去获得的基质作为支架体外构建组织工程血管时,平滑肌细胞难于迁移至支架中层,中层平滑肌密度低,植入体内6个月后中层平滑肌细胞密度仍低,平滑肌细胞有抗血管钙化作用.
배경:소구경조직공정혈관적원기결과연구겁소,상미견도연구조직공정혈관분자수평、리자수평원기결과화평활기세포여개화관계적보도.목적:이용탈세포저고동맥기질작위지가화견혈관벽세포작위충자세포체외구건소구경조직공정혈관,식입충자세포공체견고동맥부위6개월,관찰식입물중층평활기세포여개화적관계.방법:12지실험견피수궤분위지가조(n=6)화재세포화조(n=6),자체고동맥피작위대조조;지가조견접수저고동맥경탈세포후적기질지가식입쌍측고동맥,재세포화조견접수수체혈관벽세포공동배양、연합충식우탈세포적저고동맥기질병체외예괄응후식입혈관벽세포공체쌍측고동맥위치;6개월후측정식입물화대조조고동맥조직개함량、평활기밀도화병이학변화.결과여결론:소구경조직공정혈관식입후6개월검사견2조식입물무명현협착화확장,소묘전경시내표면균이완전내피화,유관벽강경화국부개화반괴형성,이상개변이지가조식입물경명현.지가조관도조직개함량현저고우재세포화조화자체고동맥(P <0.01),재세포화조식입물조직개함량역현저고우자체고동맥(P <0.01);병이학검사시재세포화조식입물평활기밀도고우지가조(P<0.01),재세포화조화지가조식입물평활기밀도균저우대조조(P<0.01);초성검사견2조관도식입술후즉각여6개월후서축폭도교린근자체고동맥서축폭도소,유부분관도무서축공능.결과제시,저고동맥상규탈세포방거획득적기질작위지가체외구건조직공정혈관시,평활기세포난우천이지지가중층,중층평활기밀도저,식입체내6개월후중층평활기세포밀도잉저,평활기세포유항혈관개화작용.
BACKGROUND:There are a few of reports about the long-term results of smal caliber tissue engineered vascular graft. There are rarely studies of relationships between molecular level of tissue engineered vascular graft, long-term results of ion level, smooth muscle cel s and calcification. OBJECTIVE:To establish the smal caliber tissue engineered vascular grafts with the decel ularized porcine femoral artery matrix as a scaffold and dog vascular wal cel s as seed cel s, and implant in femoral arterial of the seed cel donor canine for 6 months, then to study the relationships between smooth muscle cel s and calcification. METHODS:Twelve mongrels were divided into a scaffold group (n=6) and a recel ularizated scaffold group (n=6) at random. Femoral artery of mongrels was used as a control group. In the scaffold group, the decel ularizated extracel ular matrices of porcine femoral arteries were implanted in the right and left femoral arteries. Mongrels in the recel ularizated scaffold group underwent implantation of the recel ularizated extracel ular matrices in the bilateral femoral arteries established by implantation of seed cel s on the extracel ular matrices and preconditioning in vitro. Tissue calcium content, smooth muscle cel s density and pathological changes of the grafts and autologous femoral arteries were evaluated at 6 months.