CAJ | 학술논문

背景:成牙骨质细胞在牙根形成、牙周病牙骨质的再生以及正畸牙根吸收的修复中起着重要的作用,其在体外培养状态下对力学信号反应特性的变化,在国内外少见报道.目的:分析正畸牙移动张力侧及压力侧两种不同受力形式与牙骨质改建的关系.方法:采用四点弯曲细胞力学加载仪,对体外培养的成牙骨质样细胞OCCM30分别施加2000με张、压应力,应用流式细胞术检测应力刺激3,6,12,24 h后OCCM30细胞周期及增殖指数的变化.结果与结论:2000με张、压应力加载3,6 h后,成牙骨质样细胞OCCM30在S期的细胞百分率明显减小,细胞增殖活性降低;随着加力时间的延长,S期细胞百分率回升,加载24 h后S期细胞百分率与对照组无明显差异,细胞增殖活性恢复.张、压应力对增殖活性的影响趋势一致,相互之间差别无显著性意义.
배경:성아골질세포재아근형성、아주병아골질적재생이급정기아근흡수적수복중기착중요적작용,기재체외배양상태하대역학신호반응특성적변화,재국내외소견보도.목적:분석정기아이동장력측급압력측량충불동수력형식여아골질개건적관계.방법:채용사점만곡세포역학가재의,대체외배양적성아골질양세포OCCM30분별시가2000με장、압응력,응용류식세포술검측응력자격3,6,12,24 h후OCCM30세포주기급증식지수적변화.결과여결론:2000με장、압응력가재3,6 h후,성아골질양세포OCCM30재S기적세포백분솔명현감소,세포증식활성강저;수착가력시간적연장,S기세포백분솔회승,가재24 h후S기세포백분솔여대조조무명현차이,세포증식활성회복.장、압응력대증식활성적영향추세일치,상호지간차별무현저성의의.
BACKGROUND:Cementoblasts play an important role during root formation, cementum regeneration of periodontitis and repair of orthodontic root resorption. The influence of mechanical stimulation on cementoblasts in vitro is rarely reported. OBJECTIVE:To analyze the relationship between different stress patterns at tension and compression sides during orthodontic tooth movement and cementum remodeling. METHODS:2000μεtensile and compressive stress was exerted, separately, on cementoblast-like cel s OCCM30 by four-point bending system. The flow cytometry was used to examine the cel cycle and proliferation activity of OCCM30 after 3-, 6-, 12-, 24-hour loading. RESULTS AND CONCLUSION:2000μεtensile and compressive stress both depressed proliferation activity of OCCM30 through decreasing S-phase fraction after 3 and 6 hours loading. The S-phase fraction rose again with time prolonged. After 24 hours loading, the S-phase fraction and proliferation activity resumed to the level of controls. There was no significant difference between tensile and compressive stress in the influence on proliferation activity.