CAJ | 학술논문

背景:研究发现葛根素对体外骨形成具有一定的促进作用.目的:葛根素在牙周组织改建中的作用.方法:33只兔随机分成空白对照组3只、阴性对照组15只和和实验组15只.给兔双侧下颌第1磨牙安装加力装置,均加力14 d结束后,安装保持器装置,保持期对照组耳缘静脉注射生理盐水,实验组给予葛根素注射液.分别于加力保持1,3,7,14,21 d,每个时间点各处死3只,取组织标本.结果与结论:实验组:第1天远中侧可见新骨形成,边缘有成骨细胞,近中侧可见较多的破骨细胞;第14,21天近远中侧均明显的新骨堆积,致密的骨小梁形成.阴性对照组:第1天组与实验组类似;第14天近远中侧均有新骨形成,见未完全钙化的类骨质,骨吸收陷窝消失,表面见成骨细胞;第21天近远中侧均见大量新骨形成.与阴性对照组比较,各时间点实验组骨形态发生蛋白2的表达量高于阴性对照组,第7,14,21天两组表达量差异有显著性意义(P<0.05).实验组骨形态发生蛋白2的表达呈逐渐增强的趋势,阴性对照组骨形态发生蛋白2的表达呈逐渐减少的趋势.实验组与阴性对照组骨形态发生蛋白2的表达均强于空白对照组(P<0.05).提示葛根素可能在保持期通过上调骨形态发生蛋白2的表达而促进成骨细胞抑制破骨细胞从而缩短保持期时间.
배경:연구발현갈근소대체외골형성구유일정적촉진작용.목적:갈근소재아주조직개건중적작용.방법:33지토수궤분성공백대조조3지、음성대조조15지화화실험조15지.급토쌍측하합제1마아안장가력장치,균가력14 d결속후,안장보지기장치,보지기대조조이연정맥주사생리염수,실험조급여갈근소주사액.분별우가력보지1,3,7,14,21 d,매개시간점각처사3지,취조직표본.결과여결론:실험조:제1천원중측가견신골형성,변연유성골세포,근중측가견교다적파골세포;제14,21천근원중측균명현적신골퇴적,치밀적골소량형성.음성대조조:제1천조여실험조유사;제14천근원중측균유신골형성,견미완전개화적류골질,골흡수함와소실,표면견성골세포;제21천근원중측균견대량신골형성.여음성대조조비교,각시간점실험조골형태발생단백2적표체량고우음성대조조,제7,14,21천량조표체량차이유현저성의의(P<0.05).실험조골형태발생단백2적표체정축점증강적추세,음성대조조골형태발생단백2적표체정축점감소적추세.실험조여음성대조조골형태발생단백2적표체균강우공백대조조(P<0.05).제시갈근소가능재보지기통과상조골형태발생단백2적표체이촉진성골세포억제파골세포종이축단보지기시간.
BACKGROUND:Several studies have shown that puerarin has promoting effect on bone formation in vitro. OBJECTIVE:To investigate the effect of puerarin on rabbit periodontal tissue remodeling. METHODS:Total y 33 rabbits were randomly divided into the blank control group (three rabbits), the negative control group (15 rabbits) and the experimental group (15 rabbits). Rabbits’ bilateral mandibular first molars were instal ed stressing device, and after 14 days, the rabbits were instal ed retainer device. During retention period, the rabbits in the control group were injected normal saline, while the rabbits in the experimental group were injected puerarin. The two groups were maintained for 1, 3, 7, 14 and 21 days, respectively. Three rabbits were kil ed at each time point and their tissue samples were extracted. RESULTS AND CONCLUSION:In the experimental group:new bone formed in the far side, and there were osteoblasts at the verge, in addition, more osteoclasts were found in the near side at first day;at days 14 and 21, obvious accumulation of new bone was found in the near and far side, and dense trabecular bone formed. In the negative control group:at first day, it was similar to the experimental group;at day 14, there were new bones formed in the near and far side, and completely calcified osteoid was not be seen, besides, lacunar resorption disappeared, and osteoblasts were found on the surface;at day 21, a number of new bones formed in the near and far side. Compared with the negative control group, bone morphogenetic protein-2 (BMP-2) expression of the experimental group was higher at each time point. The express levels between the two groups at days 7, 14 and 21 had significant difference (P<0.05). The BMP-2 expression of the experimental group was increased gradual y, while that of the negative control group was decreased gradual y. The BMP-2 expression of the experimental group and negative control group were both higher than that of the blank control group (P<0.05). These results suggest that puerarin may promote osteoblast and inhibit osteoclast so as to shorten the retention period through the up-regulation of BMP-2 expression in retention period .