CAJ | 학술논문

背景:小鼠是常用作研究人类视网膜疾病发生、发展规律和防治措施的动物模型,视觉电生理检查已经成为检测小鼠视网膜功能的常规客观检查手段之一.目的:研制一种新型的小鼠角膜接触镜电极,并检测其在视网膜电图检查过程中的有效性和重复性.方法:首先测量不同年龄的C57BL6/J小鼠(共24只)的角膜曲率半径和角膜直径;根据所测量得到的小鼠角膜生物参数制备针对不同年龄段小鼠角膜接触镜;由数控系统车床加工获取角膜接触镜;将电极与角膜接触镜相黏附;另选取12只6周龄C57BL6/J小鼠平均分配到2组分别使用传统环状电极和角膜接触镜电极进行视网膜电图检测,分别记录暗适应和明适应视网膜电图的b波振幅,间隔1周测量1次,共3次;2周裂隙灯显微镜小鼠角膜形态.结果与结论:小鼠角膜直径在不同年龄的小鼠中具有较大差异,在2.23-3.41 mm;设计小鼠角膜接触镜的曲率为2.00 mm,直径为3.5 mm;小鼠角膜接触镜电极在测量暗适应和明适应b波振幅平均值为传统环状电极的82.7%和80.3%,而反复多次检测时,角膜接触电极稳定性高于传统环状电极;使用角膜接触镜电极检测小鼠角膜新生血管翳明显少于环形电极.表明小鼠角膜接触镜电极可以有效的应用于视觉电生理的检测,虽其测量b波振幅较传统环状电极低,但其在多次测量的稳定性较高,并且在电生理检测过程中可以有效降低小鼠角膜损伤.
배경:소서시상용작연구인류시망막질병발생、발전규률화방치조시적동물모형,시각전생리검사이경성위검측소서시망막공능적상규객관검사수단지일.목적:연제일충신형적소서각막접촉경전겁,병검측기재시망막전도검사과정중적유효성화중복성.방법:수선측량불동년령적C57BL6/J소서(공24지)적각막곡솔반경화각막직경;근거소측량득도적소서각막생물삼수제비침대불동년령단소서각막접촉경;유수공계통차상가공획취각막접촉경;장전겁여각막접촉경상점부;령선취12지6주령C57BL6/J소서평균분배도2조분별사용전통배상전겁화각막접촉경전겁진행시망막전도검측,분별기록암괄응화명괄응시망막전도적b파진폭,간격1주측량1차,공3차;2주렬극등현미경소서각막형태.결과여결론:소서각막직경재불동년령적소서중구유교대차이,재2.23-3.41 mm;설계소서각막접촉경적곡솔위2.00 mm,직경위3.5 mm;소서각막접촉경전겁재측량암괄응화명괄응b파진폭평균치위전통배상전겁적82.7%화80.3%,이반복다차검측시,각막접촉전겁은정성고우전통배상전겁;사용각막접촉경전겁검측소서각막신생혈관예명현소우배형전겁.표명소서각막접촉경전겁가이유효적응용우시각전생리적검측,수기측량b파진폭교전통배상전겁저,단기재다차측량적은정성교고,병차재전생리검측과정중가이유효강저소서각막손상.
BACKGROUND:Mice are the animal model used most commonly for studying the occurrence, development and prevention of human retinal diseases. Retinal electrophysiological tests have become one of the routine methods for the test of mouse retina function. OBJECTIVE:To develop a new kind of contact lens electrode for mouse corneal electroretinogram (ERG) recordings, and to detect its effectiveness and repeatability in the process of the test. METHODS:First, the cornea radius of curvature and the cornea diameter of 24 C57BL6/J mice at various ages were measured. Contact lens in mice of different ages were prepared according to the measured biological parameters of mouse cornea and processed by lathe digital control ed system. The electrode was then adhered to the contact lens and became a new kind of ERG electrode. Another 12 C57BL6/J mice aged 6 weeks were averagely divided into two groups and performed ERG test with traditional annular metal ic electrode and this new designed contact lens electrode, respectively. B-wave amplitudes of both dark-adapted and light-adapted ERG in the two groups were recorded every two weeks, total y three times. Cornea status of mice was evaluated by slit lamp microscope after 2 weeks. RESULTS AND CONCLUSION:There was significant difference in cornea diameter of different age mice (1.39-1.48 mm). The designed radius of curvature of the cornea was 2.00 mm and corneal diameter was 3.5 mm. The mean values of B-wave amplitudes of both dark-adapted and light-adapted ERG using the contact lens electrode were recorded 82.7%and 80.3%of those tested by the traditional annular electrode. However the stability of contact lens electrode was much higher than that of the traditional annular electrode after repeated testing. ERG test by using contact lens electrode showed that mouse corneal neovascularization in the contact lens electrode group was obviously less tha that in the traditional electrode group. These results suggest that mouse contact lens electrode can be efficiently applied in ERG test. Although its b-wave amplitude was lower than that of traditional annular electrode, its stability in repeated test was higher, and it could effectively decrease mouse c n orneal injuries.