CAJ | 학술논문

背景:根据既有研究表明,胶原纤维走向与经穴密切相关,而每个细胞至少存在两根胶原纤维以保持连接性,因此有学者推测它是人体能量传递的信号通路.目的:以经穴胶原通路假说为依据,观察胶原信号变化对骨骼肌损伤的影响,并证明电针对胶原信号的干涉作用是其促进骨骼肌修复的基本机制.方法:雄性SD大鼠按随机化原则分为3组:对照组为空白对照;模型组应用重力化打击法造备骨骼肌损伤模型;治疗组造模后采用G6850电针刺激双侧足三里穴进行干预.结果与结论:治疗组穴位区Ⅰ型胶原纤维mRNA表达量和羟脯氨酸水平低于对照组(P<0.05),其变化趋势较模型组更为平稳;治疗组血清肌酸激酶水平在治疗第7天就达到正常水平,治疗组穴位区Ⅰ型胶原纤维对比血清肌酸激酶呈现正相关.提示针刺后穴位区的Ⅰ型胶原纤维和羟脯氨酸可能在骨骼肌损伤修复中起到关键作用,从而引导了针刺信号的传输与转换过程,而且此信号传播过程始终与骨骼肌修复的核心指标肌酸激酶呈现高度正相关.
배경:근거기유연구표명,효원섬유주향여경혈밀절상관,이매개세포지소존재량근효원섬유이보지련접성,인차유학자추측타시인체능량전체적신호통로.목적:이경혈효원통로가설위의거,관찰효원신호변화대골격기손상적영향,병증명전침대효원신호적간섭작용시기촉진골격기수복적기본궤제.방법:웅성SD대서안수궤화원칙분위3조:대조조위공백대조;모형조응용중력화타격법조비골격기손상모형;치료조조모후채용G6850전침자격쌍측족삼리혈진행간예.결과여결론:치료조혈위구Ⅰ형효원섬유mRNA표체량화간포안산수평저우대조조(P<0.05),기변화추세교모형조경위평은;치료조혈청기산격매수평재치료제7천취체도정상수평,치료조혈위구Ⅰ형효원섬유대비혈청기산격매정현정상관.제시침자후혈위구적Ⅰ형효원섬유화간포안산가능재골격기손상수복중기도관건작용,종이인도료침자신호적전수여전환과정,이차차신호전파과정시종여골격기수복적핵심지표기산격매정현고도정상관.
BACKGROUND:Studies have shown that the col agen fiber orientation is closely related with meridian, and there are at least two col agen fibers in each cel in order to stay connection with each other, so scholars have speculated that it is the human energy transfer signaling pathway. OBJECTIVE:To observe the effect of col agen fibers signal changes in the recovery of the skeletal muscle injury based on the meridian col agen pathway hypothesis, and to prove that the interference effect of electroacupuncture on col agen signal is the basic mechanism to promote skeletal muscle repair. METHODS:Male Sprague Dawley rats were randomly divided into three groups:the control group was the blank control;the model group was used to establish the skeletal muscle injury model by weight combat method;rats in the treatment group received the G6850 electric acupuncture at Zusanli (ST36). RESULTS AND CONCLUSION:The expression of col agenⅠfiber mRNA and hydroxyproline level in the treatment group were lower than those in the control group (P<0.05), and the change trend was more stable in the treatment group when compared with the model group;the serum creatine kinase level in the treatment group reached to the normal level at 7 days after treatment, and there was a positive correlation between the changes of col agenⅠfibers and the serum creatine kinase level in the treatment group. The col agenⅠfibers and hydroxyproline level in the acupoint area after acupuncture may play a key role in the repair of muscle injury, thereby guiding the transmission and conversion process of acupuncture signal, and signal propagation always positively correlates with creatine kinase level during skeletal muscle repair.