CAJ | 학술논문

背景:人体大唾液腺常因受到头颈部肿瘤放射治疗、舍格伦综合征及涎腺炎等因素的影响发生腺体萎缩,目前对长期萎缩性腮腺内组织形态变化的观察仍较少.目的:观察腮腺主导管结扎诱导腮腺萎缩后的组织转归.方法:通过结扎SD大鼠右侧腮腺主导管诱导腺体萎缩,采用苏木精-伊红染色观察正常腮腺及导管结扎后0(对照),1,3,7,14,30,60 d萎缩性腮腺组织内腺泡、导管细胞的面积;免疫组织化学染色定量分析肌上皮细胞在腮腺萎缩不同时间点的数量分布变化.结果与结论:结扎腮腺主导管后腺泡细胞出现快速凋亡,至14 d时已基本消失.随着腺体萎缩,间质逐渐纤维化并伴随炎性细胞浸润,组织内形成大量导管样结构,导管面积逐渐增加,到14 d时达到顶峰,随后逐渐减少,导管样结构呈典型的双套层结构,结扎各时间点腺泡、导管面积与对照组比较差异均有显著性意义(P<0.05).结扎后7 d内肌上皮细胞数量快速增加,随后肌上皮细胞数量增长缓慢,维持在一定的范围.表明腮腺主导管结扎诱导腺体萎缩早期腺泡细胞快速消失,出现大量导管样结构,肌上皮反应性增殖,随着腺体的萎缩由导管样结构及肌上皮细胞组成“双套层”结构可能抑制腺体的进一步萎缩.
배경:인체대타액선상인수도두경부종류방사치료、사격륜종합정급연선염등인소적영향발생선체위축,목전대장기위축성시선내조직형태변화적관찰잉교소.목적:관찰시선주도관결찰유도시선위축후적조직전귀.방법:통과결찰SD대서우측시선주도관유도선체위축,채용소목정-이홍염색관찰정상시선급도관결찰후0(대조),1,3,7,14,30,60 d위축성시선조직내선포、도관세포적면적;면역조직화학염색정량분석기상피세포재시선위축불동시간점적수량분포변화.결과여결론:결찰시선주도관후선포세포출현쾌속조망,지14 d시이기본소실.수착선체위축,간질축점섬유화병반수염성세포침윤,조직내형성대량도관양결구,도관면적축점증가,도14 d시체도정봉,수후축점감소,도관양결구정전형적쌍투층결구,결찰각시간점선포、도관면적여대조조비교차이균유현저성의의(P<0.05).결찰후7 d내기상피세포수량쾌속증가,수후기상피세포수량증장완만,유지재일정적범위.표명시선주도관결찰유도선체위축조기선포세포쾌속소실,출현대량도관양결구,기상피반응성증식,수착선체적위축유도관양결구급기상피세포조성“쌍투층”결구가능억제선체적진일보위축.
BACKGROUND:The human major salivary glands are usual y affected by various factors, such as radiation therapy, Sjogren's syndrome and sialadenitis, which can lead to atrophy. At present, there are only few studies on the morphological changes of long-term atrophic parotid gland. OBJECTIVE:To investigate the changes of the atrophic parotid gland induced by its main duct ligation. METHODS:After the duct ligation of the atrophic parotid gland induced by its right main duct of SD rats, the area of inner acinar and duct cel s of normal parotid gland and atrophic parotid gland tissues were examined by hematoxylin-eosin staining after duct ligation for 0 (control), 1, 3, 7, 14, 30 and 60 days. Changes in number and distribution of myoepithelial cel s in the atrophic parotid gland at different time points were analyzed by immunohistochemical staining method. RESULTS AND CONCLUSION:Morphological analysis showed that rapid apoptosis in the acinar cel s appeared after the ligation of the main duct in the parotid gland, and cel s almost disappeared at day 14. With the atrophy of the parotid gland, a gradual decrease and fibrosis of the glandular lobules with inflammatory cel infiltration appeared. And a number of duct-like structures formed. Besides, the area of duct was gradual y increased, and then was gradual y decreased after reaching the peak at day 14. Duct-like structures showed a classic double-layers structure. The areas of acinar and duct in the experimental group were significantly different from those in the control group (P<0.05). At day 7 after ligation, quantitative analysis showed significant increase in the number of myoepithelial cel s, fol owed by gradual increases, which, however, were not statistical y significant. These results suggest that in the earlier atrophic stage, there was a rapid disappearance of acinar accompanied by the occurrence of duct-like structures, and reactive myoepithelial cel s proliferation. Ultimately, the“double-layers”structures composed by myoepithelial cel s and duct-like structures may have great significances in the process of resisting atrophy.