CAJ | 학술논문

背景:苯妥英钠在创面愈合过程中的抑菌作用仍不明确.目的:通过体外及动物实验观察苯妥英钠在创面愈合过程中是否具有抑菌作用.方法:①体外实验:采用水解酪蛋白肉汤稀释法检测苯妥英钠的最低抑菌浓度、琼脂扩散法观察1024 mg/L的苯妥英钠的体外抑菌活性.②动物实验:将30只SD大鼠随机等分为3组,在大鼠背侧脊柱旁设计一2 cm×3 cm矩形创面,分别用10,20 mg/cm2苯妥英钠糊剂或单纯凡士林纱布进行处理,创面均外加凡士林油纱及干纱包扎固定.隔日换药,于第4,8,12,16天进行创面表面细菌定量培养.结果与结论:①水解酪蛋白肉汤稀释法结果:应用质控菌株、临床菌株进行试验,不同苯妥英钠浓度的试管中均呈现浑浊状态.②琼脂扩散法结果:应用质控菌株进行试验,未发现抑菌圈的形成.③实验动物创面细菌定量培养结果:应用10,20 mg/cm2苯妥英钠糊剂处理的创面细菌定量略低于单纯凡士林纱布处理的创面,但各组之间差异均无显著性意义(P>0.05).表明苯妥英钠尚无明确的体外及体内抑菌活性,对创面细菌的清除无明显作用.
배경:분타영납재창면유합과정중적억균작용잉불명학.목적:통과체외급동물실험관찰분타영납재창면유합과정중시부구유억균작용.방법:①체외실험:채용수해락단백육탕희석법검측분타영납적최저억균농도、경지확산법관찰1024 mg/L적분타영납적체외억균활성.②동물실험:장30지SD대서수궤등분위3조,재대서배측척주방설계일2 cm×3 cm구형창면,분별용10,20 mg/cm2분타영납호제혹단순범사림사포진행처리,창면균외가범사림유사급간사포찰고정.격일환약,우제4,8,12,16천진행창면표면세균정량배양.결과여결론:①수해락단백육탕희석법결과:응용질공균주、림상균주진행시험,불동분타영납농도적시관중균정현혼탁상태.②경지확산법결과:응용질공균주진행시험,미발현억균권적형성.③실험동물창면세균정량배양결과:응용10,20 mg/cm2분타영납호제처리적창면세균정량략저우단순범사림사포처리적창면,단각조지간차이균무현저성의의(P>0.05).표명분타영납상무명학적체외급체내억균활성,대창면세균적청제무명현작용.
BACKGROUND:The antibacterial activity of phenytoin in wound healing process is stil not clear. OBJECTIVE:To observe the antibacterial activity of phenytoin in the wound healing process through in vitro and animal experiment. METHODS: ①In vitro experiment:Casein hydrolyzate broth dilution was used to observe the minimum inhibitory concentration, and agar diffusion method was used to observe in vitro antibacterial activity of 1.024 mg/L phenytoin.②Animal experiments:Thirty Sprague Dawley rats were divided into three equal groups;a 2 cmx3 cm rectangular wound was created in rat dorsal paraspinal, and then treated with 10 or 20 mg/cm2 phenytoin paste or pure Vaseline gauze, the wound was bandaged and fixed with Vaseline yarn and dry gauze. The dressing was changed every day, and the wound surface bacteria were quantitative cultured at 4, 8, 12 and 16 days. RESULTS AND CONCLUSION: ①Casein broth dilution method results:The quality control strains and clinical isolates test were performed, the test tubes of different phenytoin concentration showed a turbid state. ②Agar diffusion method results:The quality control strains test was performed, and no inhibition zone formation was observed. ③Experimental animal wound bacteria quantitative culture results:The bacteria quantization in the group treated with 10 or 20 mg/cm2 phenytoin paste was lower than that in the group treated with pure Vaseline gauze, but there was no significant difference between groups (P>0.05). The results show that the in vitro or in vivo antibacterial activity of phenytoin is not clear, and it has no significant effect on the wound bacterial clearance.