CAJ | 학술논문

背景:早期实验证实骨膜含有潜在形成软骨或骨的间充质干细胞,在适当的条件下可向软骨细胞分化.目的:比较观察多孔钽支架复合骨膜移植与磷酸三钙复合骨膜移植修复软骨缺损的效果.方法:取雌性兔24只随机分为2组.建立膝关节软骨缺损模型,分别填入多孔钽支架和磷酸三钙支架,表面覆盖预处理的反置骨膜.石膏固定2周.于12周麻醉后处死兔,观察滑膜、关节液、股骨髁软骨大体观及股骨髁软骨病理表现.采用改良的Mankin骨关节炎的评分法.结果与结论:多孔钽组滑膜增生明显,新生软骨表层呈蓝白色,周缘欠光滑,甲苯胺蓝染色可见软骨细胞排列稍紊乱,软骨细胞数目正常,多孔钽内骨长入良好,Mankin评分为7.35分.磷酸三钙组新生软骨表层呈蓝白色,周缘欠光滑,甲苯胺蓝染色可见软骨细胞排列稍紊乱,软骨细胞数目正常,磷酸三钙内骨长入可,Mankin评分为7.43分(P>0.05).表明多孔钽支架复合骨膜移植与磷酸三钙复合骨膜移植修复方式对软骨修复的结果无明显差别,但多孔钽支架与周围骨组织融合优于磷酸三钙.
배경:조기실험증실골막함유잠재형성연골혹골적간충질간세포,재괄당적조건하가향연골세포분화.목적:비교관찰다공단지가복합골막이식여린산삼개복합골막이식수복연골결손적효과.방법:취자성토24지수궤분위2조.건립슬관절연골결손모형,분별전입다공단지가화린산삼개지가,표면복개예처리적반치골막.석고고정2주.우12주마취후처사토,관찰활막、관절액、고골과연골대체관급고골과연골병리표현.채용개량적Mankin골관절염적평분법.결과여결론:다공단조활막증생명현,신생연골표층정람백색,주연흠광활,갑분알람염색가견연골세포배렬초문란,연골세포수목정상,다공단내골장입량호,Mankin평분위7.35분.린산삼개조신생연골표층정람백색,주연흠광활,갑분알람염색가견연골세포배렬초문란,연골세포수목정상,린산삼개내골장입가,Mankin평분위7.43분(P>0.05).표명다공단지가복합골막이식여린산삼개복합골막이식수복방식대연골수복적결과무명현차별,단다공단지가여주위골조직융합우우린산삼개.
BACKGROUND:Early experiments have proved that synovium contains mesenchymal stem cel s which can potential y form the cartilage or bone and differentiate into chondrocytes under property conditions. OBJECTIVE:To compare the repair effect of porous tantalum combined with synovium and tricalcium phosphate combined with synovium on cartilage defects. METHODS:Total y 24 female New Zealand rabbits were randomly divided into experimental group (a porous tantalum scaffold combined with synovium) and control group (a tricalcium phosphate scaffold combined with synovium). A model of cartilage defect in the knee joint was establishe, and then the two kinds of scaffolds were implanted. A piece of pretreated inverse synovium was covered on the surface of defects, and plaster fixation was performed for 2 weeks. The rabbits were executed under anesthesia at week 12. The gross observation of the synovium, joint fluid and femoral condylar cartilage, as wel as the pathological features of the femoral condylar cartilage was observed. Improved Mankin score was obtained to evaluate the repair effect. RESULTS AND CONCLUSION:In the experimental group, there was various synovial hyperplasia, and the surface of new cartilage was blue and white, and the margin was less smooth. Toluidine blue staining showed that chondrocytes arranged a little disorder. Resides, the number of chondrocytes was normal, moreover the porous tantalum scaffolds were ful y fil ed with bone tissues, and the Mankin score was 7.35. In the control group, the surface of new cartilage was blue and white, and the margin was less smooth. Toluidine blue staining showed that chondrocytes arranged a little disorderly. The number of chondrocytes was normal. In addition, the tricalcium phosphate scaffolds were partly fil ed with bone, and the Mankin score was 7.43 (P>0.05). These findings suggest that there is no significant difference between porous tantalum combined with synovium and tricalcium phosphate combined with synovium in repairing cartilage defects, but the fusion of porous tantalum scaffolds with surrounding bone tissues is superior to that of tricalcium phosphate.