CAJ | 학술논문

背景:细胞在支架上的生长行为受到支架孔径及孔隙率等多种因素影响.目的:观察在体外中空钛合金假体壁上孔洞直径对骨髓基质干细胞生长及分化的影响.方法:将中空多孔金属试件按照壁上及底面孔径大小分为:φ1:1 mm;φ2:1.5 mm两组,均置于骨髓基质干细胞悬液中进行成骨诱导培养,以未成骨诱导培养作对照.分别于2,4周倒置相差显微镜观察细胞形态、生长情况,成骨诱导钙结节结果.结果与结论:4周时φ1实验组孔洞中细胞相向生长并相连,φ2组孔洞中未见相连现象;成骨诱导组在第4周有明显的钙结节形成.表明在体外骨髓基质干细胞在适当的钛合金金属孔径内可直接相向生长并联结,以预防植入物-宿主骨潜在间隙的形成;同时这种空间构型可为再生骨提供足够的生长空间.
배경:세포재지가상적생장행위수도지가공경급공극솔등다충인소영향.목적:관찰재체외중공태합금가체벽상공동직경대골수기질간세포생장급분화적영향.방법:장중공다공금속시건안조벽상급저면공경대소분위:φ1:1 mm;φ2:1.5 mm량조,균치우골수기질간세포현액중진행성골유도배양,이미성골유도배양작대조.분별우2,4주도치상차현미경관찰세포형태、생장정황,성골유도개결절결과.결과여결론:4주시φ1실험조공동중세포상향생장병상련,φ2조공동중미견상련현상;성골유도조재제4주유명현적개결절형성.표명재체외골수기질간세포재괄당적태합금금속공경내가직접상향생장병련결,이예방식입물-숙주골잠재간극적형성;동시저충공간구형가위재생골제공족구적생장공간.
BACKGROUND:The growth behavior of cel s on a stent is influenced by the pore size and porosity of stents as wel as many other factors. OBJECTIVE:To observe the effect of different pore sizes of hol ow titanium al oy prosthesis’s wal on the growth of bone marrow stromal cel s (BMSCs). METHODS:Hol ow porous metal prostheses were divided into two groups by the pore size of the wal , which wereφ1:1 mm andφ2:1.5 mm. The prosthesis was located in the BMSCs suspension for osteogenic induction. BMSCs without osteogenic induction served as controls. The cel morphology, growth and calcium nodules were observed under inverted phase contrast microscope. RESULTS AND CONCLUSION:At 4 week, BMSCs were linked directly through the hole of the groupφ1, but not in the groupφ2. Calcium nodules were formed in the groupsφ1 andφ2. These indicate that BMSCs cultured in vitro can be linked directly in the proper pore size to prevent a potential gap between implants and the host bone. In addition, this spatial structure can provide sufficient space for the growth of regenerated bones.