CAJ | 학술논문

背景:小肠黏膜下层细胞外基质材料免疫原性低,具有良好生物相容性,是构建单一结构工程化组织的较好支架材料.目的:观察体外兔骨髓间充质干细胞与猪小肠黏膜下层复合培养的生物相容性.方法:采用密度梯度离心法结合贴壁法分离纯化培养兔骨髓间充质干细胞,在其接种前用红色免疫荧光标记,然后将第2代已标记的兔骨髓间充质干细胞接种在猪小肠黏膜下层上.结果与结论:①组织学观察:骨髓间充质干细胞与小肠黏膜下层上复合培养1周时细胞呈单层生长,荧光显微镜下观察标记的骨髓间充质干细胞显示均匀红色荧光,复合培养2周细胞呈多层生长,并显示更密集的红色荧光.②扫描电镜观察:复合培养2 d,骨髓间充质干细胞黏附于材料表面并伸展;复合培养1周,小肠黏膜下层被骨髓间充质干细胞分泌的胶原覆盖;2周后细胞在材料上已大量增殖形成融合,细胞连接紧密,细胞分泌大量基质,并分层.表明小肠黏膜下层与骨髓间充质干细胞具有良好的相容性.
배경:소장점막하층세포외기질재료면역원성저,구유량호생물상용성,시구건단일결구공정화조직적교호지가재료.목적:관찰체외토골수간충질간세포여저소장점막하층복합배양적생물상용성.방법:채용밀도제도리심법결합첩벽법분리순화배양토골수간충질간세포,재기접충전용홍색면역형광표기,연후장제2대이표기적토골수간충질간세포접충재저소장점막하층상.결과여결론:①조직학관찰:골수간충질간세포여소장점막하층상복합배양1주시세포정단층생장,형광현미경하관찰표기적골수간충질간세포현시균균홍색형광,복합배양2주세포정다층생장,병현시경밀집적홍색형광.②소묘전경관찰:복합배양2 d,골수간충질간세포점부우재료표면병신전;복합배양1주,소장점막하층피골수간충질간세포분비적효원복개;2주후세포재재료상이대량증식형성융합,세포련접긴밀,세포분비대량기질,병분층.표명소장점막하층여골수간충질간세포구유량호적상용성.
BACKGROUND:Smal intestinal submucosa extracel ular matrix is a kind of material used to build the single structural engineering scaffolds with low immune response and good biocompatibility. OBJECTIVE:To observe the biocompatibility of bone marrow mesenchymal stem cel s growing on the smal intestinal submucosa in vitro. METHODS:The primary bone marrow mesenchymal stem cel s were separated and purified by density gradient centrifugation combined with adherent methods. The cel s were labeled using the red fluorescent cel linker kit before seeding. The passage 2 labeled bone marrow mesenchymal stem cel s were seeded on the smal intestinal submucosa. RESULTS AND CONCLUSION:①H istologicalobservation showed that the bone marrow mesenchymal stem cel s formed a single layer on the surface of smal intestinal submucosa after coculture for 1 week. The labeled bone marrow mesenchymal stem cel s on the scaffold showed uniformly red fluorescence by fluorescence microscope. Two weeks later, the cel s were in multi-layer, and displayed intensively red fluorescence. ②Scanning electron microscope showed that, after 2 days of coculture, bone marrow mesenchymal stem cel s were adherent to the smal intestinal submucosa;1 week later, the smal intestinal submucosa was covered with the col agen secreted by bone marrow mesenchymal stem cel s;2 weeks later, the cel s proliferated and fused on co-cultured smal intestinal submucosa and closely connected, which showed stratifications and secreted a large number of matrix. The data obtained from the present study shows that bone marrow msenchymal stem cel s and smal intestinal submucosa have a good compatibility.