CAJ | 학술논문

背景:丝素蛋白纤维材料具有透明性、结构可塑性、成分单一性、力学强韧性及生物相容性等特点.目的:综述国内外丝素蛋白应用于角膜组织工程的研究进展.方法:由第一作者在标题和中以“silk fibroin, corneal, ocular”或“丝素,角膜”为检索词,检索1980至2011年PubMed及1990至2011年CNKI数据库中关于丝素蛋白角膜的文章.结果与结论:从天然蚕丝中提取的高分子丝素蛋白,因其良好的生物相容性、独特的力学性能、光学透明性及降解速率可控性,既可以单独应用于角膜组织结构的重建,又可与其他组织材料联合应用,成为角膜组织工程学应用的理想材料.现已证明多种角膜细胞可在丝素纤维膜上良好生长,但体外培养的细胞应用于动物模型的相关研究较少;此外丝素蛋白材料植入角膜内对其产生何种影响的研究数据较缺乏,这些均是亟待解决的问题.
배경:사소단백섬유재료구유투명성、결구가소성、성분단일성、역학강인성급생물상용성등특점.목적:종술국내외사소단백응용우각막조직공정적연구진전.방법:유제일작자재표제화중이“silk fibroin, corneal, ocular”혹“사소,각막”위검색사,검색1980지2011년PubMed급1990지2011년CNKI수거고중관우사소단백각막적문장.결과여결론:종천연잠사중제취적고분자사소단백,인기량호적생물상용성、독특적역학성능、광학투명성급강해속솔가공성,기가이단독응용우각막조직결구적중건,우가여기타조직재료연합응용,성위각막조직공정학응용적이상재료.현이증명다충각막세포가재사소섬유막상량호생장,단체외배양적세포응용우동물모형적상관연구교소;차외사소단백재료식입각막내대기산생하충영향적연구수거교결핍,저사균시극대해결적문제.
BACKGROUND:Silk fibroin has the properties of pel ucidness, structural plasticity, single component, mechanical strength, toughness and biocompatibility. OBJECTIVE:To review the research progress of silk fibroin as a scaffold material for corneal tissue engineering at home and abroad. METHODS:Databases of PubMed (1980/2011) and CNKI (1990/2011) were selected to retrieve the related articles about silk fibroin and cornea with the keywords of“silk fibroin, corneal, ocular”in English and Chinese, respectively. RESULTS AND CONCLUSION:Owing to the good properties of silk fibroin extracted from nature silk, such as good biocompatibility, special mechanical property, optic transparency and control ed degradation rate, the silk fibroin cannot only be applied in the reconstruction of corneal tissue structure, but also be combined with other tissue materials. The silk fibroin has become an ideal material for corneal tissue engineering. It has been proved that many kinds of corneal cel s can grow wel on the silk fibroin, and however, there are rare studies about application of cel s cultured in vitro in animal models. In addition, there are few data addressing the influence of implantation of silk fibroin into the cornea, which are problems to be solved.