CAJ | 학술논문

背景:研究证实力学刺激是影响骨改建的重要因素,可促进骨髓基质干细胞骨向分化;但不同幅度力学刺激对骨髓基质干细胞分化的影响尚不明确. 目的:观察持续张应力对大鼠骨髓基质干细胞成骨分化的影响. 方法:全血贴壁培养法获取大鼠骨髓基质干细胞.采用 Flexercell-4000细胞体外应力加载系统对骨髓基质干细胞施加5%,10%,15%幅度的持续张应力,对照组则不加力培养,频率1 Hz,持续时间48 h.分别在加力后1,6,12,24,48 h 检测成骨标记物碱性磷酸酶、Ⅰ型胶原、骨钙素 mRNA 及成骨特异性转录因子 Runx2的 mRNA 及蛋白表达. 结果与结论:5%和10%持续张应力作用下,骨髓基质干细胞的成骨标记基因碱性磷酸酶、Ⅰ型胶原、骨钙素 mRNA的表达较对照组升高(P <0.05),10%张力组升高的时间均较5%张力组早、幅度较高.15%持续在加力6 h 时可促进骨髓基质干细胞碱性磷酸酶、Ⅰ型胶原 mRNA 的表达(P <0.05)、随后表达下降,加力48 h 后上述指标均低于对照组(P <0.05),骨钙素 mRNA 的表达加力6 h 后均低于对照组(P <0.05).5%张力组仅加力24 h 后骨髓基质干细胞 Runx2蛋白表达高于对照组(P <0.05),10%,15%张力组加力6 h 后 Runx2蛋白表达均高于对照组(P <0.05).结果证实,5%,10%,15%持续张应力均可更有效地促进骨髓基质干细胞的骨向分化,10%持续张力的促进效应更显著.
배경:연구증실역학자격시영향골개건적중요인소,가촉진골수기질간세포골향분화;단불동폭도역학자격대골수기질간세포분화적영향상불명학. 목적:관찰지속장응력대대서골수기질간세포성골분화적영향. 방법:전혈첩벽배양법획취대서골수기질간세포.채용 Flexercell-4000세포체외응력가재계통대골수기질간세포시가5%,10%,15%폭도적지속장응력,대조조칙불가력배양,빈솔1 Hz,지속시간48 h.분별재가력후1,6,12,24,48 h 검측성골표기물감성린산매、Ⅰ형효원、골개소 mRNA 급성골특이성전록인자 Runx2적 mRNA 급단백표체. 결과여결론:5%화10%지속장응력작용하,골수기질간세포적성골표기기인감성린산매、Ⅰ형효원、골개소 mRNA적표체교대조조승고(P <0.05),10%장력조승고적시간균교5%장력조조、폭도교고.15%지속재가력6 h 시가촉진골수기질간세포감성린산매、Ⅰ형효원 mRNA 적표체(P <0.05)、수후표체하강,가력48 h 후상술지표균저우대조조(P <0.05),골개소 mRNA 적표체가력6 h 후균저우대조조(P <0.05).5%장력조부가력24 h 후골수기질간세포 Runx2단백표체고우대조조(P <0.05),10%,15%장력조가력6 h 후 Runx2단백표체균고우대조조(P <0.05).결과증실,5%,10%,15%지속장응력균가경유효지촉진골수기질간세포적골향분화,10%지속장력적촉진효응경현저.
BACKGROUND: Mechanical stimulation can promote the osteogenic differentiation of bone marrow stromal cel s, but the effect of different magnitude of tensile stress on the bone marrow stromal cel s is not clear. @@@@OBJECTIVE: To study the effect of different magnitudes of continuous tensile stress on the osteogenic differentiation of rat bone marrow stromal cel s. @@@@METHODS: Rat bone marrow stromal cel s were obtained and purified by ful -blood attachment culture. The 5%, 10%and 15% continuous tensile stress (1 Hz) were strained on bone marrow stromal cel s with Flexercel -4000 mechanical loading system and lasted for 48 hours, and the cel s in the control group were cultured without stress. The mRNA and protein expression of osteoblast-related genes alkaline phosphatase, col agen type Ⅰ, osteocalcin and osteoblast-specific transcription factor Runx2 were analyzed at 1, 6, 12, 24 and 48 hours after strain. @@@@RESULTS AND CONCLUSION: The mRNA expression of alkaline phosphatase, col agen type Ⅰ and osteocalcin was increased in rat bone marrow stromal cel s subjected to 5% and 10% continuous tensile stress when compared with the control group (P < 0.05), but the 10% tensile stress group increased earlier than the 5% tensile stress group, and the amplitude was higher. The mRNA expression of alkaline phosphatase and col agen type Ⅰ was increased at 6&nbsp