CAJ | 학술논문

背景:髋关节置换后假体的融合率与假体周围的骨重建密切相关,这个过程中骨髓间充质干细胞的募集、向成骨细胞分化的途径受骨形态发生蛋白调控. 目的:分析股骨近端 Stro-1+细胞与骨形态发生蛋白7、骨形态发生蛋白受体的相关性. 方法:32例患者初次进行髋关节置换,取术中股骨矩开槽时骨块,体外细胞培养14 d 后检测骨髓间充质干细胞数量, Stro-1+标记干细胞的总量,骨形态发生蛋白7、骨形态发生蛋白受体1a、骨形态发生蛋白受体2的表达. 结果与结论:Stro-1+、骨形态发生蛋白7、骨形态发生蛋白受体1a、骨形态发生蛋白受体2的表达在不同年龄组有轻微差异,但差异无显著性意义,在不同性别组差异也无显著性意义;股骨近端 Stro-1+细胞数量(即代表具有向成骨细胞分化功能的细胞)与骨形态发生蛋白7 表达呈负相关关系,与骨形态发生蛋白受体1a 表达呈正相关关系,其可以影响髋关节假体的骨融合与生存率.
배경:관관절치환후가체적융합솔여가체주위적골중건밀절상관,저개과정중골수간충질간세포적모집、향성골세포분화적도경수골형태발생단백조공. 목적:분석고골근단 Stro-1+세포여골형태발생단백7、골형태발생단백수체적상관성. 방법:32례환자초차진행관관절치환,취술중고골구개조시골괴,체외세포배양14 d 후검측골수간충질간세포수량, Stro-1+표기간세포적총량,골형태발생단백7、골형태발생단백수체1a、골형태발생단백수체2적표체. 결과여결론:Stro-1+、골형태발생단백7、골형태발생단백수체1a、골형태발생단백수체2적표체재불동년령조유경미차이,단차이무현저성의의,재불동성별조차이야무현저성의의;고골근단 Stro-1+세포수량(즉대표구유향성골세포분화공능적세포)여골형태발생단백7 표체정부상관관계,여골형태발생단백수체1a 표체정정상관관계,기가이영향관관절가체적골융합여생존솔.
BACKGROUND: A successful osseointegration after total hip arthroplasty relies on the bone reconstruction around the prosthesis. Bone marrow mesenchymal stem cel s are recruited and the commitment of bone marrow mesenchymal stem cel s to an osteoblastic differentiation pathway is apparently under the control of bone morphogenic proteins. OBJECTIVE: To analyze the correlation between Stro-1+ bone marrow mesenchymal stem cel s and bone morphogenetic protein 7 and bone morphogenetic protein receptor in proximal femur. @@@@METHODS: Bone samples were obtained from the discarded metaphysis region of the proximal femur in 32 patients with primary total hip arthroplasty, and the number of bone marrow mesenchymal stem cel s was measured after in vitro cultured for 14 days. Stro-1+ was used to mark the stem cel s. The expression of bone morphogenetic protein 7, bone morphogenetic protein receptor 1a and bone morphogenetic protein receptor 2 was detected. @@@@RESULTS AND CONCLUSION: There were differences of Stro-1+ cel s, bone morphogenetic protein 7, bone morphogenetic protein receptor 1a and bone morphogenetic protein receptor 2 expression between different years, but the differences were not significant, and there was no significant difference between genders. There was a negative correlation between the numbers of Stro-1+ cel s in proximal femur and expression of bone morphogenetic protein 7 that means the cel had the function to differentiate into osteoblasts. The correlation between the number of Stro-1+ cel s and expression of bone morphogenetic protein receptor 1a was positive which can influence the fusion rate and the survival rate of the hip prosthesis.