CAJ | 학술논문

背景:脐带间充质干细胞在成脂培养条件下成脂相关信号因子过氧化物酶体增殖体激活受体γ的表达水平增高,同时成骨相关信号因子骨形态发生蛋白的表达水平也增高. 目的:检测成脂诱导条件下脐带间充质干细胞的过氧化物酶体增殖体激活受体γ及骨形态发生蛋白2的表达水平变化,并对结果进行初步探讨. 方法:取脐带来源的间充质干细胞,以成脂诱导体系对细胞进行诱导分化培养,倒置显微镜下观察细胞体外培养生长情况;油红 O 染色法观察成脂现象,茜素红及 von kossa 染色观察是否有沉淀形成;荧光定量 PCR 方法检测成脂相关因子过氧化物酶体增殖体激活受体γ及骨形态发生蛋白2的表达变化情况. 结果与结论:获得脐带来源间充质干细胞,并成功进行了成脂诱导分化.荧光定量 PCR 检测发现在成脂诱导条件下脐带间充质干细胞的过氧化物酶体增殖体激活受体γ和骨形态发生蛋白2的表达水平全都呈升高趋势,但是前者对分化方向的调控起主要作用.
배경:제대간충질간세포재성지배양조건하성지상관신호인자과양화물매체증식체격활수체γ적표체수평증고,동시성골상관신호인자골형태발생단백적표체수평야증고. 목적:검측성지유도조건하제대간충질간세포적과양화물매체증식체격활수체γ급골형태발생단백2적표체수평변화,병대결과진행초보탐토. 방법:취제대래원적간충질간세포,이성지유도체계대세포진행유도분화배양,도치현미경하관찰세포체외배양생장정황;유홍 O 염색법관찰성지현상,천소홍급 von kossa 염색관찰시부유침정형성;형광정량 PCR 방법검측성지상관인자과양화물매체증식체격활수체γ급골형태발생단백2적표체변화정황. 결과여결론:획득제대래원간충질간세포,병성공진행료성지유도분화.형광정량 PCR 검측발현재성지유도조건하제대간충질간세포적과양화물매체증식체격활수체γ화골형태발생단백2적표체수평전도정승고추세,단시전자대분화방향적조공기주요작용.
BACKGROUND: Expression of bone morphogenetic protein 2 and peroxisome proliferator-activated receptorγgene in umbilical cord mesenchymal stem cel s after adipogenic induction is increased at the same time. @@@@OBEJECTIVE: To investigate the changes of bone morphogenetic protein 2 and peroxisome proliferator-activated @@@@receptorγgene expression in umbilical cord mesenchymal stem cel s after adipogenic induction, and to compare the results. METHODS: With specific inductive medium, umbilical cord mesenchymal stem cel s were obtained and then induced into adipogenisis. Their morphological characteristics were observed with inverted microscope. Oil red O staining was used to observe the adipogenic phenomenon, and von kossa staining and alizarin red staining were used to identify the formation of precipitation. Real-time polymerase chain reaction was used to quantify the gene expression of bone morphogenetic protein 2 and peroxisome proliferator-activated receptor γ at various time points after adipogenic induction. @@@@RESULTS AND CONCLUSION: Umbilical cord mesenchymal stem cel s could be isolated and cultured successful y. After cultured in adipogenic medium, the cel s differentiated into adipogenisis. The result of real-time polymerase chain reaction showed that both the expression levels of bone morphogenetic protein 2 and peroxisome proliferator-activated receptorγgene were increased after adipogenic induction, but peroxisome proliferator-activated receptorγplayed a major role in the regulation of differentiation.