CAJ | 학술논문

背景:碱性成纤维细胞生长因子作为一种具有强烈促进新生血管生成和脂肪细胞增殖分化作用的细胞因子,在临床上广泛应用于自体脂肪移植术. 目的:观察不同质量浓度的碱性成纤维细胞生长因子对脂肪来源干细胞促增殖的影响,分析促进脂肪来源干细胞增殖的适合浓度. 方法:从抽脂术得到的脂肪组织当中分离培养脂肪来源干细胞,观察细胞形态及生长情况.分别配制含有0,5,10,20,40,80,160μg/L 碱性成纤维细胞生长因子的培养液培养脂肪干细胞.连续7 d 应用 MTT 法检测脂肪来源干细胞的增殖. 结果与结论:碱性成纤维细胞生长因子有明显促进脂肪来源干细胞增殖的作用,第3天开始出现明显的促增殖作用,第5,6天达到高峰.实验组各质量浓度之间对脂肪来源干细胞的促增殖作用无明显差异,周围环境中碱性成纤维细胞生长因子的质量浓度达到5μg/L 即可对脂肪来源干细胞起到明显的促进作用,增加剂量促增殖作用无明显改变.
배경:감성성섬유세포생장인자작위일충구유강렬촉진신생혈관생성화지방세포증식분화작용적세포인자,재림상상엄범응용우자체지방이식술. 목적:관찰불동질량농도적감성성섬유세포생장인자대지방래원간세포촉증식적영향,분석촉진지방래원간세포증식적괄합농도. 방법:종추지술득도적지방조직당중분리배양지방래원간세포,관찰세포형태급생장정황.분별배제함유0,5,10,20,40,80,160μg/L 감성성섬유세포생장인자적배양액배양지방간세포.련속7 d 응용 MTT 법검측지방래원간세포적증식. 결과여결론:감성성섬유세포생장인자유명현촉진지방래원간세포증식적작용,제3천개시출현명현적촉증식작용,제5,6천체도고봉.실험조각질량농도지간대지방래원간세포적촉증식작용무명현차이,주위배경중감성성섬유세포생장인자적질량농도체도5μg/L 즉가대지방래원간세포기도명현적촉진작용,증가제량촉증식작용무명현개변.
BACKGROUND: Basic fibroblast growth factors have been widely used for clinical autologous fat grafting because they exhibit great potential to promote angiogenesis and adipocyte proliferation and differentiation. OBJECTIVE: To investigate the effects of different concentrations of basic fibroblast growth factors on proliferation of adipose-derived stem cel s and analyze the proper concentration of basic fibroblast growth factors for promoting proliferation of adipose-derived stem cel s. METHODS: Adipose-derived stem cel s were isolated from adipose tissue by liposuction. Cel morphology and growth characteristics were observed under the microscope. Culture medium containing 0, 5, 10, 20, 40, 80, 60 μg/L basic fibroblast growth factors was prepared. Adipose-derived stem cel s were cultured in a 96-wel plate at 103 cel s/wel using culture medium containing basic fibroblast growth factors. The proliferation of adipose-derived stem cel s was determined by MTT for successive 7 days. RESULTS AND CONCLUSION: Basic fibroblast growth factors could promote the proliferation of adipose-derived stem cel s significantly. The proliferation-promoting effect was significant since the 3rd day and peaked on the 5th and 6th days. There was no obvious difference in proliferation-promoting effects between different basic fibroblast growth factor concentrations. 5 μg/L was the most appropriate concentration for basic fibroblast growth factors in promoting the proliferation of adipose-derived stem cel s.