CAJ | 학술논문

背景:目前,对不同来源肝干细胞的分化潜能尚未形成一致意见,在运用中也无统一标准和规范.目的:综述不同来源肝干细胞定向分化的潜能. 方法:应用计算机检索1990年1月至2011年11月 PubMed 数据库相关文章,检索词为“differentiation,hepatic stem cel ,liver stem cel ”,并限定文章语言种类为 English.同时计算机检索1990年1月至2011年11月中国知网数据库相关文章,检索词为“肝干细胞,分化”,并限定文章语言种类为中文.最终纳入符合标准的文献42篇.结果与结论:绝大多数肝干细胞的基础研究集中在胚胎来源肝干细胞或卵圆细胞,而在临床研究中,脐血、骨髓等来源肝干细胞获得了更多的关注,业内对各来源肝干细胞的分化潜能尚未形成一致意见.文章综述不同来源肝干细胞分化潜能的差异,探讨不同诱导因子在其中的决定作用,从而为进一步筛选稳定可靠的诱导因子,改善诱导定向分化的稳定性打下基础.同时期待更多符合临床需求的基础研究支持肝干细胞的应用,在肝干细胞来源和诱导剂上逐步形成一致的共识,建立统一的干细胞采集标准,甚至临床操作规范.
배경:목전,대불동래원간간세포적분화잠능상미형성일치의견,재운용중야무통일표준화규범.목적:종술불동래원간간세포정향분화적잠능. 방법:응용계산궤검색1990년1월지2011년11월 PubMed 수거고상관문장,검색사위“differentiation,hepatic stem cel ,liver stem cel ”,병한정문장어언충류위 English.동시계산궤검색1990년1월지2011년11월중국지망수거고상관문장,검색사위“간간세포,분화”,병한정문장어언충류위중문.최종납입부합표준적문헌42편.결과여결론:절대다수간간세포적기출연구집중재배태래원간간세포혹란원세포,이재림상연구중,제혈、골수등래원간간세포획득료경다적관주,업내대각래원간간세포적분화잠능상미형성일치의견.문장종술불동래원간간세포분화잠능적차이,탐토불동유도인자재기중적결정작용,종이위진일보사선은정가고적유도인자,개선유도정향분화적은정성타하기출.동시기대경다부합림상수구적기출연구지지간간세포적응용,재간간세포래원화유도제상축보형성일치적공식,건립통일적간세포채집표준,심지림상조작규범.
BACKGROUND: The hepatic stem cel s originated from embryo or hepatic oval cel s were always studied actively in basic researches. But the other stem cel s from umbilical cord blood or bone marrow are paid more attention in clinical study. There is no agreement on differentiation of varied hepatic stem cel s. OBJECTIVE: To summarize the potentiality of directed differentiation of hepatic stem cel s from different origins. METHODS: PubMed databases and China National Knowledge Infrastructure (CNKI) were retrieved for articles published from January 1990 to October 2011 in English and Chinese respectively with the searching words of differentiation, hepatic stem cel or liver stem cel . Articles in the same research field published in the authoritative journals or recently published (past 3 years) were included. Forty-two articles were included in the final analysis. RESULTS AND CONCLUSION: The majority of basic studies of hepatic stem cel s focus on embryo-derived hepatic stem cel s or hepatic oval cel s; while in the clinical studies, umbilical cord- and bone marrow-derived hepatic stem cel s have been paid more attention. There is no agreement on differentiation potential of hepatic stem cel s from various origins. This paper summarizes the difference of differentiation potential of hepatic stem cel s from different origins and investigates the decisive effect of different inductors so as to provide strong evidence for further screening reliable inductors and improving directed induced differentiation. More basic studies involving hepatic stem cel s are expected to meet clinical requirements. This would help to achieve agreement in origins and inductors of hepatic stem cel s and establish uniform standards to col ect stem cel s or uniform clinical operation guideline.