CAJ | 학술논문

背景:咖啡酸对破骨细胞形成分化方面影响的研究很少,对破骨细胞性骨吸收机制的研究更少.目的:观察咖啡酸对破骨细胞形成分化及组织蛋白酶 K 基因表达的影响,分析甘薯提取物咖啡酸对破骨细胞性骨吸收的抑制机制.方法:采用3种不同细胞培养系,观察咖啡酸对破骨细胞形成分化的影响,同时利用反转录-聚合酶链反应技术观察咖啡酸对组织蛋白酶 K 基因表达的抑制作用.结果与结论:咖啡酸在3种不同细胞培养系中均显示出直接抑制破骨细胞形成和分化的作用,根据半定量反转录-聚合酶链反应基因表达分析,咖啡酸对 RANKL 受体 RANK mRNA 的表达完全无影响,但显著抑制了组织蛋白酶 K的表达.提示甘薯提取物有效成分咖啡酸不仅能直接抑制破骨细胞的形成及分化,而且可显著抑制组织蛋白酶 K 的表达,其可能是甘薯提取物抑制破骨细胞性骨吸收的作用机制.
배경:가배산대파골세포형성분화방면영향적연구흔소,대파골세포성골흡수궤제적연구경소.목적:관찰가배산대파골세포형성분화급조직단백매 K 기인표체적영향,분석감서제취물가배산대파골세포성골흡수적억제궤제.방법:채용3충불동세포배양계,관찰가배산대파골세포형성분화적영향,동시이용반전록-취합매련반응기술관찰가배산대조직단백매 K 기인표체적억제작용.결과여결론:가배산재3충불동세포배양계중균현시출직접억제파골세포형성화분화적작용,근거반정량반전록-취합매련반응기인표체분석,가배산대 RANKL 수체 RANK mRNA 적표체완전무영향,단현저억제료조직단백매 K적표체.제시감서제취물유효성분가배산불부능직접억제파골세포적형성급분화,이차가현저억제조직단백매 K 적표체,기가능시감서제취물억제파골세포성골흡수적작용궤제.
BACKGROUND: To date, there are few researches on the effect of caffeic acid on the formation and differentiation of osteoclasts, and fewer researches on the mechanism of osteoclastic bone resorption. OBJECTIVE: To explore the mechanism of sweet potato extract caffeic acid inhibiting osteoclastic bone resorption by studying the effect of caffeic acid on the formation and differentiation of osteoclast and on the expression of cathepsin K gene. METHODS: Caffeic acid effects on formation and differentiation of osteoclasts in three osteoclast cel lines were observed and the inhibition effect of caffeic acid on the expression of cathepsin K gene was detected by reverse transcription-PCR. RESULTS AND CONCLUSION: Caffeic acid could directly inhibit the formation and differentiation of osteoclasts in three osteoclast cel lines. Caffeic acid had no influences on the expression of receptor activator of nuclear factor-kappa B ligand and receptor activator of nuclear factor-kappa B mRNA, but could markedly inhibit the expression of cathepsin K gene based on the semi-quantitative gene analysis of reverse transcription-PCR. Caffeic acid, an effective component of sweet potato extract, cannot only directly inhibit the formation and differentiation of osteoclast, but also markedly inhibit the expression of cathepsin K gene, which seems to be the mechanism of sweet potato extract inhibiting osteoclastic bone resorption.